PMID- 17434051
OWN - NLM
STAT- MEDLINE
DCOM- 20070511
LR  - 20131121
IS  - 1590-3729 (Electronic)
IS  - 0939-4753 (Linking)
VI  - 17
IP  - 4
DP  - 2007 May
TI  - Erythrocyte transmembrane electron transfer in haemodialysis patients.
PG  - 288-93
AB  - BACKGROUND AND AIMS: Patients with chronic renal failure, especially those 
      treated with haemodialysis, have an increased risk of developing atherosclerotic 
      vascular disease probably as a result of enhanced oxidative stress. The human 
      cell membrane possesses electron transfer systems which protect against 
      extracellular pro-oxidant challenge. We evaluated (1) the erythrocyte velocity of 
      ferricyanide reduction (RBC vfcy) in 25 uraemic patients (aged 25-71 years; 14 
      males), (2) the changes induced by a single haemodialysis session and (3) 
      biomarkers of oxidative stress. METHODS AND RESULTS: Before and after a mid-week 
      dialysis session, we measured RBC vfcy, erythrocyte glutathione (RBC GSH), plasma 
      and red cell membrane malondialdehyde (P and RBC MDA), plasma sulphydryl groups 
      (P SH), plasma vitamin C levels and haemolysis percentage. Pre-dialysis RBC GSH 
      (0.68+/-0.13 vs 0.80+/-0.13 mg/mL, p<0.01), P SH (266+/-74 vs 406+/-78 
      micromol/L, p<0.01) and plasma vitamin C (7.0+/-5.1 vs 21.5+/-8.5mg/L, p<0.001) 
      were lower than in 25 age-sex-matched healthy controls; P MDA (1.57+/-0.52 vs 
      0.54+/-0.29 nmol/mL, p<0.001), RBC MDA (0.42+/-0.13 vs 0.34+/-0.16 nmol/mL, 
      p<0.05) and haemolysis (1.2+/-0.3 vs 0.7+/-0.3%, p<0.001) were increased. 
      Baseline RBC vfcy did not differ from normals (13.1+/-5.2 vs 12.9+/-3.2 
      mmol/mL/h). Following dialysis, RBC vfcy (to 8.9+/-4.5 mmol/mL/h, p<0.001) 
      decreased, as well as P MDA, RBC MDA and plasma vitamin C (to 2.5+/-1.4 mg/L, 
      p<0.001), whereas P SH groups increased (to 413+/-99 micromol/L, p<0.001); 
      haemolysis percentage remained high. RBC vfcy values were correlated to RBC GSH 
      and vitamin C levels. CONCLUSIONS: Uraemic patients showed signs of oxidative 
      stress. Pre-dialysis RBC vfcy is maintained in the normal range on account of a 
      reduced intracellular content of GSH and in spite of low plasma ascorbate. A 
      single haemodialysis treatment reduced biomarkers of protein and lipid oxidation 
      but markedly impaired transmembrane electron transfer, which could be explained 
      by acute depletion of electron donors.
FAU - Matteucci, Elena
AU  - Matteucci E
AD  - Department of Internal Medicine, University of Pisa, Via Rome 67, 56126 Pisa, 
      Italy. ematteuc@int.med.unipi.it
FAU - Cupisti, Adamasco
AU  - Cupisti A
FAU - Caprioli, Raffaele
AU  - Caprioli R
FAU - Battipaglia, Elena
AU  - Battipaglia E
FAU - Favilla, Stefania
AU  - Favilla S
FAU - Rindi, Paolo
AU  - Rindi P
FAU - Barsotti, Giuliano
AU  - Barsotti G
FAU - Giampietro, Ottavio
AU  - Giampietro O
LA  - eng
PT  - Journal Article
DEP - 20060320
PL  - Netherlands
TA  - Nutr Metab Cardiovasc Dis
JT  - Nutrition, metabolism, and cardiovascular diseases : NMCD
JID - 9111474
RN  - 0 (Free Radicals)
RN  - 0 (Sulfhydryl Compounds)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Ascorbic Acid/metabolism
MH  - Electron Transport
MH  - Erythrocyte Membrane/*metabolism
MH  - Female
MH  - Free Radicals
MH  - Humans
MH  - Male
MH  - Malondialdehyde/blood
MH  - Middle Aged
MH  - Oxidation-Reduction
MH  - *Oxidative Stress
MH  - *Renal Dialysis
MH  - Sulfhydryl Compounds/blood
EDAT- 2007/04/17 09:00
MHDA- 2007/05/12 09:00
CRDT- 2007/04/17 09:00
PHST- 2005/05/23 00:00 [received]
PHST- 2005/11/24 00:00 [revised]
PHST- 2005/11/24 00:00 [accepted]
PHST- 2007/04/17 09:00 [pubmed]
PHST- 2007/05/12 09:00 [medline]
PHST- 2007/04/17 09:00 [entrez]
AID - S0939-4753(05)00255-3 [pii]
AID - 10.1016/j.numecd.2005.11.011 [doi]
PST - ppublish
SO  - Nutr Metab Cardiovasc Dis. 2007 May;17(4):288-93. doi: 
      10.1016/j.numecd.2005.11.011. Epub 2006 Mar 20.