PMID- 17434154
OWN - NLM
STAT- MEDLINE
DCOM- 20070727
LR  - 20240312
IS  - 0008-8749 (Print)
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 244
IP  - 2
DP  - 2006 Dec
TI  - A systematic bioinformatics approach for selection of epitope-based vaccine 
      targets.
PG  - 141-7
AB  - Epitope-based vaccines provide a new strategy for prophylactic and therapeutic 
      application of pathogen-specific immunity. A critical requirement of this 
      strategy is the identification and selection of T-cell epitopes that act as 
      vaccine targets. This study describes current methodologies for the selection 
      process, with dengue virus as a model system. A combination of publicly available 
      bioinformatics algorithms and computational tools are used to screen and select 
      antigen sequences as potential T-cell epitopes of supertype human leukocyte 
      antigen (HLA) alleles. The selected sequences are tested for biological function 
      by their activation of T-cells of HLA transgenic mice and of pathogen infected 
      subjects. This approach provides an experimental basis for the design of pathogen 
      specific, T-cell epitope-based vaccines that are targeted to majority of the 
      genetic variants of the pathogen, and are effective for a broad range of 
      differences in human leukocyte antigens among the global human population.
FAU - Khan, Asif M
AU  - Khan AM
AD  - Department of Microbiology, Yong Loo Lin School of Medicine, National University 
      of Singapore, 5 Science Drive 2, Singapore 117597, Singapore.
FAU - Miotto, Olivo
AU  - Miotto O
FAU - Heiny, A T
AU  - Heiny AT
FAU - Salmon, Jerome
AU  - Salmon J
FAU - Srinivasan, K N
AU  - Srinivasan KN
FAU - Nascimento, Eduardo J M
AU  - Nascimento EJ
FAU - Marques, Ernesto T A Jr
AU  - Marques ET Jr
FAU - Brusic, Vladimir
AU  - Brusic V
FAU - Tan, Tin Wee
AU  - Tan TW
FAU - August, J Thomas
AU  - August JT
LA  - eng
GR  - U19 AI056541/AI/NIAID NIH HHS/United States
GR  - U19 AI056541-05/AI/NIAID NIH HHS/United States
GR  - 5 U19 AI56541/AI/NIAID NIH HHS/United States
GR  - HHSN2662-00400085C/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070416
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA Antigens)
RN  - 0 (Viral Nonstructural Proteins)
RN  - 0 (Viral Vaccines)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Computational Biology/*methods
MH  - Conserved Sequence
MH  - Dengue/*immunology/prevention & control
MH  - Dengue Virus/genetics/*immunology
MH  - Epitopes, T-Lymphocyte/genetics/*immunology
MH  - HLA Antigens/genetics/*immunology
MH  - Humans
MH  - Interferon-gamma/immunology
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Viral Nonstructural Proteins/genetics/immunology
MH  - Viral Vaccines/genetics/*immunology
PMC - PMC2041846
MID - NIHMS24122
EDAT- 2007/04/17 09:00
MHDA- 2007/07/28 09:00
PMCR- 2007/10/24
CRDT- 2007/04/17 09:00
PHST- 2007/01/31 00:00 [received]
PHST- 2007/02/06 00:00 [accepted]
PHST- 2007/04/17 09:00 [pubmed]
PHST- 2007/07/28 09:00 [medline]
PHST- 2007/04/17 09:00 [entrez]
PHST- 2007/10/24 00:00 [pmc-release]
AID - S0008-8749(07)00026-3 [pii]
AID - 10.1016/j.cellimm.2007.02.005 [doi]
PST - ppublish
SO  - Cell Immunol. 2006 Dec;244(2):141-7. doi: 10.1016/j.cellimm.2007.02.005. Epub 
      2007 Apr 16.