PMID- 17434169
OWN - NLM
STAT- MEDLINE
DCOM- 20070815
LR  - 20081121
IS  - 0016-6480 (Print)
IS  - 0016-6480 (Linking)
VI  - 152
IP  - 2-3
DP  - 2007 Jun-Jul
TI  - The influence of ploidy on saltwater adaptation, acute stress response and immune 
      function following seawater transfer in non-smolting rainbow trout.
PG  - 314-25
AB  - We investigated the effect of ploidy on osmoregulatory, stress and immune 
      responses in non-smolting rainbow trout during saltwater adaptation. Sibling 
      groups of diploid and triploid trout were acclimated in freshwater (FW) and then 
      subjected to abrupt transfer to full strength (35ppt) saltwater (SW) or back to 
      FW. Fish were sampled pre-stress, and 1, 3, 6, 12, 24, 48, 72 and 168h 
      post-stress. Overall mortality in SW was less than 5% in either ploidy, with no 
      mortality in FW. Significant elevations in plasma osmolality and gill ATPase were 
      observed within 1-3h of SW transfer, but retuned to basal levels within 72h 
      indicative of rapid saltwater adaptation and did not differ between ploidy. 
      Furthermore, FW-SW transfer also caused significant and sustained elevations in 
      total blood haemoglobin, plasma IGF-I, cortisol, glucose, total white blood cell 
      counts, increased plasma but decreased mucus lysozyme, and enhanced head kidney 
      macrophage respiratory burst activity. Conversely, FW-FW transfer evoked more 
      transient and less elevated responses, more typical of primary and secondary 
      responses to a single stressor. We conclude that the more elevated levels in 
      these parameters are a function of saltwater adaptation as well as the generic 
      stress response, and that this did not differ between ploidy. Strong positive 
      correlations were found between plasma IGF-I and cortisol, and with osmolality, 
      glucose and WBC, while a negative correlation was found with plasma lysozyme 
      irrespective of ploidy. Overall, the current results suggest that triploidy does 
      not affect the ability of non-smolting trout to adapt to full strength seawater 
      under optimum conditions, and that the osmotic and stress response to such 
      transfer is similar to diploids.
FAU - Taylor, J F
AU  - Taylor JF
AD  - Institute of Aquaculture, University of Stirling, Stirling, Scotland FK9 4LA, UK. 
      jft2@stir.ac.uk
FAU - Needham, M P
AU  - Needham MP
FAU - North, B P
AU  - North BP
FAU - Morgan, A
AU  - Morgan A
FAU - Thompson, K
AU  - Thompson K
FAU - Migaud, H
AU  - Migaud H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070302
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (Hemoglobins)
SB  - IM
MH  - Adaptation, Physiological/*physiology
MH  - Animals
MH  - Diploidy
MH  - Endocrine System/physiology
MH  - Female
MH  - Hematocrit
MH  - Hemoglobins
MH  - Immune System/physiology
MH  - Leukocyte Count
MH  - Oncorhynchus mykiss/*genetics/growth & development
MH  - *Ploidies
MH  - Seawater
MH  - Stress, Physiological/*genetics/*immunology/physiopathology
MH  - Survival Rate
MH  - Water-Electrolyte Balance/*physiology
EDAT- 2007/04/17 09:00
MHDA- 2007/08/19 09:00
CRDT- 2007/04/17 09:00
PHST- 2006/09/14 00:00 [received]
PHST- 2007/02/23 00:00 [revised]
PHST- 2007/02/24 00:00 [accepted]
PHST- 2007/04/17 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2007/04/17 09:00 [entrez]
AID - S0016-6480(07)00091-3 [pii]
AID - 10.1016/j.ygcen.2007.02.029 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2007 Jun-Jul;152(2-3):314-25. doi: 
      10.1016/j.ygcen.2007.02.029. Epub 2007 Mar 2.