PMID- 17434557
OWN - NLM
STAT- MEDLINE
DCOM- 20070801
LR  - 20181113
IS  - 0042-6822 (Print)
IS  - 0042-6822 (Linking)
VI  - 364
IP  - 2
DP  - 2007 Aug 1
TI  - Ebola virus-like particle-induced activation of NF-kappaB and Erk signaling in 
      human dendritic cells requires the glycoprotein mucin domain.
PG  - 342-54
AB  - Dendritic cells (DCs), important early targets of Ebola virus (EBOV) infection in 
      vivo, are activated by Ebola virus-like particles (VLPs). To better understand 
      this phenomenon, we have systematically assessed the response of DCs to VLPs of 
      different compositions. VLPs containing the viral matrix protein (VP40) and the 
      viral glycoprotein (GP), were found to induce a proinflammatory response highly 
      similar to a prototypical DC activator, LPS. This response included the 
      production of several proinflammatory cytokines, activation of numerous 
      transcription factors including NF-kappaB, the functional importance of which was 
      demonstrated by employing inhibitors of NF-kappaB activation, and activation of 
      ERK1/2 MAP kinase. In contrast, VLPs constituted with a mutant GP lacking the 
      heavily glycosylated mucin domain showed impaired NF-kappaB and Erk activation 
      and induced less DC cytokine production. We conclude that the GP mucin domain is 
      required for VLPs to stimulate human dendritic cells through NF-kappaB and MAPK 
      signaling pathways.
FAU - Martinez, Osvaldo
AU  - Martinez O
AD  - Department of Microbiology, Box 1124, Mount Sinai School of Medicine, 1 Gustave L 
      Levy Place, New York, NY 10029, USA.
FAU - Valmas, Charalampos
AU  - Valmas C
FAU - Basler, Christopher F
AU  - Basler CF
LA  - eng
GR  - U19 AI062623/AI/NIAID NIH HHS/United States
GR  - U19 AI062623-030002/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070416
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Cytokines)
RN  - 0 (IL6 protein, human)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-6)
RN  - 0 (Mucins)
RN  - 0 (NF-kappa B)
RN  - 0 (Transcription Factors)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/immunology/*metabolism/*virology
MH  - Ebolavirus/genetics/*pathogenicity/physiology/ultrastructure
MH  - Extracellular Signal-Regulated MAP Kinases/*metabolism
MH  - Humans
MH  - Inflammation Mediators/metabolism
MH  - Interleukin-6/biosynthesis
MH  - MAP Kinase Signaling System
MH  - Microscopy, Electron
MH  - Mucins/chemistry/genetics
MH  - NF-kappa B/*metabolism
MH  - Protein Structure, Tertiary
MH  - Transcription Factors/metabolism
MH  - Transfection
MH  - Viral Proteins/chemistry/genetics/*physiology
MH  - Virion/genetics/pathogenicity/physiology/ultrastructure
PMC - PMC2034500
MID - NIHMS25784
EDAT- 2007/04/17 09:00
MHDA- 2007/08/02 09:00
PMCR- 2008/08/01
CRDT- 2007/04/17 09:00
PHST- 2006/11/22 00:00 [received]
PHST- 2007/01/05 00:00 [revised]
PHST- 2007/03/09 00:00 [accepted]
PHST- 2007/04/17 09:00 [pubmed]
PHST- 2007/08/02 09:00 [medline]
PHST- 2007/04/17 09:00 [entrez]
PHST- 2008/08/01 00:00 [pmc-release]
AID - S0042-6822(07)00178-X [pii]
AID - 10.1016/j.virol.2007.03.020 [doi]
PST - ppublish
SO  - Virology. 2007 Aug 1;364(2):342-54. doi: 10.1016/j.virol.2007.03.020. Epub 2007 
      Apr 16.