PMID- 17434565
OWN - NLM
STAT- MEDLINE
DCOM- 20070904
LR  - 20070528
IS  - 0043-1354 (Print)
IS  - 0043-1354 (Linking)
VI  - 41
IP  - 12
DP  - 2007 Jun
TI  - Natural organic matter (NOM) removal and structural changes in the bacterial 
      community during artificial groundwater recharge with humic lake water.
PG  - 2715-25
AB  - This study evaluated the removal of natural organic matter (NOM) and structural 
      changes in the microbial community during infiltration of humic lake water at 
      three artificial groundwater recharge (AGR) sites in Finland. The three sites 
      were at waterworks in Hameenlinna, Jyvaskyla and Tuusula, sites A, B and C, 
      respectively. Site A used groundwater recharge by both basin and sprinkling 
      infiltration, site B used only sprinkling infiltration, and site C used only 
      basin infiltration. Reductions of total organic carbon at sites A, B and C were 
      91%, 84% and 74%, respectively, in the winter, and 88%, 77% and 73%, 
      respectively, in the summer. The Finnish national recommended value of 2 mg/l for 
      TOC was achieved at all sites and the TOC of natural groundwater at site C was 
      much lower, at 0.6 mg/l. Large molecular fractions of NOM were removed more 
      efficiently than the smaller ones. Total amount of DAPI-stained cells decreased 
      during infiltration at sites A, B and C in winter by 94%, 94% and 75% and in 
      summer by 96%, 97% and 94%, respectively. Bacterial communities in raw waters and 
      extracted groundwaters were diverse with changes occurring during infiltration, 
      which was shown by DNA extraction followed by PCR of 16S rRNA genes and 
      denaturing gradient gel electrophoresis (DGGE) fingerprinting. While the natural 
      groundwater microbial community was diverse, it was different from that of the 
      extracted groundwater in the AGR area. Simultaneous organic carbon removal and 
      the decrease of bacterial counts during infiltration indicated biodegradation. In 
      addition, the changing DGGE profiles during the process of infiltration, 
      demonstrated that changing environmental conditions were reflected by changes in 
      bacterial community composition.
FAU - Kolehmainen, Reija E
AU  - Kolehmainen RE
AD  - Institute of Environmental Engineering and Biotechnology, Tampere University of 
      Technology, P.O. Box 541, FIN-33101 Tampere, Finland. reija.kolehmainen@tut.fi
FAU - Langwaldt, Jorg H
AU  - Langwaldt JH
FAU - Puhakka, Jaakko A
AU  - Puhakka JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070416
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (Humic Substances)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Water Pollutants)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Bacteria/genetics/isolation & purification
MH  - Carbon/analysis
MH  - Colony Count, Microbial
MH  - Finland
MH  - Fresh Water/analysis/microbiology
MH  - *Humic Substances
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Seasons
MH  - *Water Microbiology
MH  - Water Pollutants/*analysis
MH  - Water Supply/*analysis
EDAT- 2007/04/17 09:00
MHDA- 2007/09/05 09:00
CRDT- 2007/04/17 09:00
PHST- 2006/06/30 00:00 [received]
PHST- 2006/12/19 00:00 [revised]
PHST- 2007/02/22 00:00 [accepted]
PHST- 2007/04/17 09:00 [pubmed]
PHST- 2007/09/05 09:00 [medline]
PHST- 2007/04/17 09:00 [entrez]
AID - S0043-1354(07)00158-3 [pii]
AID - 10.1016/j.watres.2007.02.042 [doi]
PST - ppublish
SO  - Water Res. 2007 Jun;41(12):2715-25. doi: 10.1016/j.watres.2007.02.042. Epub 2007 
      Apr 16.