PMID- 17436000
OWN - NLM
STAT- MEDLINE
DCOM- 20080108
LR  - 20181113
IS  - 0172-8172 (Print)
IS  - 0172-8172 (Linking)
VI  - 27
IP  - 11
DP  - 2007 Sep
TI  - Activin A suppresses interleukin-1-induced matrix metalloproteinase 3 secretion 
      in human chondrosarcoma cells.
PG  - 1049-55
AB  - The objective was to investigate the effect of activin A on matrix 
      metalloproteinase 3 (MMP-3) production and to identify the role of activin A in 
      chondroprotection. SW1353 cells, a human chondrosarcoma cell line, were 
      stimulated with interleukin (IL) 1alpha and tumor necrosis factor (TNF) alpha, 
      and the concentrations of activin A, follistatin, and MMP-3 secreted into the 
      culture media were measured by enzyme-linked immunosorbent assay (ELISA). Activin 
      A was added to cell cultures in the presence of IL-1alpha or TNFalpha to 
      determine its effect on the production of MMP-3 and sulfated glycosaminoglycan 
      (sGAG) (measured by Alcian blue assay). To study the mechanism responsible for 
      the chondroprotective effects of activin A, the production of IL-1 receptor 
      antagonist (IL-1ra) and tissue inhibitor for metalloproteinases 1 (TIMP-1) was 
      examined by ELISA. Addition of IL-1alpha did not affect the production of activin 
      A by cultured SW1353 cells. IL-1alpha and activin A inhibited the production of 
      follistatin. Stimulation of SW1353 cells with activin A suppressed 
      IL-1alpha-induced, but not TNFalpha-induced, MMP-3 expression. Activin A had no 
      effect on the production of sGAG, IL-1ra, or TIMP-1, although it suppressed the 
      induction of TIMP-1 and IL-1ra by IL-1alpha. This novel finding of MMP-3 
      inhibition by activin A suggests a new role of activin A in cartilage remodeling. 
      Activin A may have therapeutic potential for preventing cartilage degradation.
FAU - Chang, Deh-Ming
AU  - Chang DM
AD  - Department of Rheumatology, Immunology and Allergy, Tri-Service General Hospital, 
      National Defense Medical Center, #325 Cheng-Kung Road, Section 2, Neihu 114, 
      Taipei, Taiwan. ming0503@ms3.hinet.net
FAU - Liu, Shao-Hsiang
AU  - Liu SH
FAU - Lee, Herng-Sheng
AU  - Lee HS
FAU - Lai, Jenn-Hung
AU  - Lai JH
FAU - Chen, Chen-Hung
AU  - Chen CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070414
PL  - Germany
TA  - Rheumatol Int
JT  - Rheumatology international
JID - 8206885
RN  - 0 (Follistatin)
RN  - 0 (Interleukin-1alpha)
RN  - 0 (activin A)
RN  - 104625-48-1 (Activins)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Activins/*physiology
MH  - Cell Line, Tumor
MH  - Chondrocytes/*metabolism
MH  - Chondrosarcoma/*metabolism
MH  - Follistatin/metabolism
MH  - Humans
MH  - Interleukin-1alpha/*physiology
MH  - Matrix Metalloproteinase 3/*metabolism
EDAT- 2007/04/17 09:00
MHDA- 2008/01/09 09:00
CRDT- 2007/04/17 09:00
PHST- 2006/10/28 00:00 [received]
PHST- 2007/03/25 00:00 [accepted]
PHST- 2007/04/17 09:00 [pubmed]
PHST- 2008/01/09 09:00 [medline]
PHST- 2007/04/17 09:00 [entrez]
AID - 10.1007/s00296-007-0350-5 [doi]
PST - ppublish
SO  - Rheumatol Int. 2007 Sep;27(11):1049-55. doi: 10.1007/s00296-007-0350-5. Epub 2007 
      Apr 14.