PMID- 17440720
OWN - NLM
STAT- MEDLINE
DCOM- 20070904
LR  - 20131121
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 75
IP  - 3
DP  - 2007 Jun
TI  - Quantitative analysis of a high-rate hydrogen-producing microbial community in 
      anaerobic agitated granular sludge bed bioreactors using glucose as substrate.
PG  - 693-701
AB  - Fermentative H(2) production microbial structure in an agitated granular sludge 
      bed bioreactor was analyzed using fluorescence in situ hybridization (FISH) and 
      polymerase chain reaction-denatured gradient gel electrophoresis (PCR-DGGE). This 
      hydrogen-producing system was operated at four different hydraulic retention 
      times (HRTs) of 4, 2, 1, and 0.5 h and with an influent glucose concentration of 
      20 g chemical oxygen demand/l. According to the PCR-DGGE analysis, bacterial 
      community structures were mainly composed of Clostridium sp. (possibly 
      Clostridium pasteurianum), Klebsiella oxytoca, and Streptococcus sp. Significant 
      increase of Clostridium/total cell ratio (68%) was observed when the reactor was 
      operated under higher influent flow rate. The existence of Streptococcus sp. in 
      the reactor became more important when operated under a short HRT as indicated by 
      the ratio of Streptococcus probe-positive cells to Clostridium probe-positive 
      cells changing from 21% (HRT 4 h) to 38% (HRT 0.5 h). FISH images suggested that 
      Streptococcus cells probably acted as seeds for self-flocculated granule 
      formation. Furthermore, combining the inspections with hydrogen production under 
      different HRTs and their corresponding FISH analysis indicated that K. oxytoca 
      did not directly contribute to H(2) production but possibly played a role in 
      consuming O(2) to create an anaerobic environment for the hydrogen-producing 
      Clostridium.
FAU - Hung, Chun-Hsiung
AU  - Hung CH
AD  - Department of Environmental Engineering, National Chung-Hsing University, 
      Taichung, 402, Taiwan. badger@nchu.edu.tw
FAU - Lee, Kuo-Shing
AU  - Lee KS
FAU - Cheng, Lu-Hsiu
AU  - Cheng LH
FAU - Huang, Yu-Hsin
AU  - Huang YH
FAU - Lin, Ping-Jei
AU  - Lin PJ
FAU - Chang, Jo-Shu
AU  - Chang JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070418
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 7YNJ3PO35Z (Hydrogen)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Bacteria, Anaerobic/classification/genetics/*metabolism
MH  - Bioreactors/*microbiology
MH  - Electrophoresis/methods
MH  - Fermentation
MH  - Glucose/*metabolism
MH  - Hydrogen/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
EDAT- 2007/04/19 09:00
MHDA- 2007/09/05 09:00
CRDT- 2007/04/19 09:00
PHST- 2006/12/07 00:00 [received]
PHST- 2007/01/21 00:00 [accepted]
PHST- 2007/01/18 00:00 [revised]
PHST- 2007/04/19 09:00 [pubmed]
PHST- 2007/09/05 09:00 [medline]
PHST- 2007/04/19 09:00 [entrez]
AID - 10.1007/s00253-007-0854-7 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2007 Jun;75(3):693-701. doi: 
      10.1007/s00253-007-0854-7. Epub 2007 Apr 18.