PMID- 17441795
OWN - NLM
STAT- MEDLINE
DCOM- 20070717
LR  - 20220330
IS  - 0105-4538 (Print)
IS  - 1398-9995 (Electronic)
IS  - 0105-4538 (Linking)
VI  - 62
IP  - 5
DP  - 2007 May
TI  - Isolation and characterization of native Cry j 3 from Japanese cedar (Cryptomeria 
      japonica) pollen.
PG  - 547-53
AB  - BACKGROUND: Japanese cedar (Cryptomeria japonica) pollinosis is the most 
      prevalent allergy in Japan. Recently, the Japanese cedar pollen allergen Cry j 3 
      was cloned as a homologue of Jun a 3, which is a major allergen from mountain 
      cedar (Juniperus ashei) pollen. However, native Cry j 3 has not been isolated and 
      there are no reports on its allergenic activity. The aims of this study were to 
      isolate native Cry j 3 and assess its immunoglobulin E (IgE)-binding capacity in 
      patients with Japanese cedar pollinosis. METHODS: Native Cry j 3 was purified 
      from Japanese cedar pollen by multidimensional chromatography. We assessed the 
      IgE-binding capacity using sera from patients allergic to Japanese cedar pollen 
      by immunoblot analysis and ELISA. Moreover, we assayed the capacity of Cry j 3 to 
      induce histamine release from the patients' leukocytes. We cloned cDNA 
      corresponding to purified Cry j 3 from a cDNA library of Japanese cedar pollen. 
      RESULTS: We isolated native Cry j 3 as a 27-kDa protein. The IgE-binding 
      frequency of Cry j 3 from the sera of patients allergic to Japanese cedar pollen 
      was estimated as 27% (27/100) by ELISA. Cry j 3 induced the release of histamine 
      from leukocytes. We cloned the cDNA and named it Cry j 3.8. Cry j 3.8 cDNA 
      encoded 225 amino acids and had significant homology with thaumatin-like 
      proteins. CONCLUSIONS: Cry j 3 is a causative allergen in Japanese cedar 
      pollinosis and may play crucial roles in the cross-reactivity with oral allergy 
      syndrome.
FAU - Fujimura, T
AU  - Fujimura T
AD  - Graduate School of Medicine, Chiba University, Chiba, Japan.
FAU - Futamura, N
AU  - Futamura N
FAU - Midoro-Horiuti, T
AU  - Midoro-Horiuti T
FAU - Togawa, A
AU  - Togawa A
FAU - Goldblum, R M
AU  - Goldblum RM
FAU - Yasueda, H
AU  - Yasueda H
FAU - Saito, A
AU  - Saito A
FAU - Shinohara, K
AU  - Shinohara K
FAU - Masuda, K
AU  - Masuda K
FAU - Kurata, K
AU  - Kurata K
FAU - Sakaguchi, M
AU  - Sakaguchi M
LA  - eng
GR  - K08 AI055792/AI/NIAID NIH HHS/United States
GR  - K08 AI055792-01A1/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Denmark
TA  - Allergy
JT  - Allergy
JID - 7804028
RN  - 0 (Antigens, Plant)
RN  - 0 (Cry j 3 allergen, Cryptomeria japonica)
RN  - 0 (Protein Isoforms)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antigens, Plant/immunology/*isolation & purification/metabolism
MH  - Base Sequence
MH  - Binding Sites, Antibody
MH  - Cryptomeria/chemistry/genetics/*immunology
MH  - Humans
MH  - Hypersensitivity/immunology/metabolism
MH  - Immunoglobulin E/biosynthesis/blood
MH  - Molecular Sequence Data
MH  - Pollen/chemistry/*immunology
MH  - Protein Binding/immunology
MH  - Protein Isoforms/immunology/metabolism
PMC - PMC2587458
MID - NIHMS79492
EDAT- 2007/04/20 09:00
MHDA- 2007/07/18 09:00
PMCR- 2008/11/25
CRDT- 2007/04/20 09:00
PHST- 2007/04/20 09:00 [pubmed]
PHST- 2007/07/18 09:00 [medline]
PHST- 2007/04/20 09:00 [entrez]
PHST- 2008/11/25 00:00 [pmc-release]
AID - ALL1331 [pii]
AID - 10.1111/j.1398-9995.2007.01331.x [doi]
PST - ppublish
SO  - Allergy. 2007 May;62(5):547-53. doi: 10.1111/j.1398-9995.2007.01331.x.