PMID- 17444617
OWN - NLM
STAT- MEDLINE
DCOM- 20070613
LR  - 20081121
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 46
IP  - 19
DP  - 2007 May 15
TI  - Properties of nucleosomes in acetylated mouse mammary tumor virus versus 5S 
      arrays.
PG  - 5623-34
AB  - Acetylation is one of the most abundant histone modifications found in 
      nucleosomes. Although such modifications are thought to function mainly in 
      recognition, acetylation is known to produce nucleosome structural alterations. 
      These could be of functional significance in vivo. Here, the basic features of 
      mouse mammary tumor virus (MMTV) promoter nucleosomal arrays reconstituted with 
      highly acetylated histones prepared from butyrate-treated HeLa cells are 
      characterized by atomic force microscopy. Results are compared to previous 
      results obtained with hypoacetylated MMTV and hyper- or hypoacetylated 5S rDNA 
      arrays. MMTV arrays containing highly acetylated histones show diminished 
      intramolecular compaction compared to hypoacetylated MMTV arrays and no tendency 
      for cooperativity in nucleosome occupation. Both features have been suggested to 
      reflect histone tail-mediated internucleosomal interactions; these observations 
      are consistent with that suggestion. 5S arrays show qualitatively similar 
      behavior. Two other effects of acetylation show stronger DNA template dependence. 
      Nucleosome salt stability is diminished in highly acetylated compared to 
      hypoacetylated MMTV arrays, but nucleosome (histone) loading tendencies are 
      unaffected by acetylation. However, highly acetylated histones show reduced 
      loading tendencies on 5S templates (vs hypoacetylated), but 5S nucleosome salt 
      stabilities are unaffected by acetylation. ATP-dependent nucleosome remodeling by 
      human Swi-Snf is similar on hyper- and hypoacetylated MMTV arrays.
FAU - Solis, F J
AU  - Solis FJ
AD  - Department of Integrated Natural Life Sciences, Arizona State University West, 
      Glendale, Arizona 85306, USA.
FAU - Bash, R
AU  - Bash R
FAU - Wang, H
AU  - Wang H
FAU - Yodh, J
AU  - Yodh J
FAU - Lindsay, S A
AU  - Lindsay SA
FAU - Lohr, D
AU  - Lohr D
LA  - eng
GR  - CA-85990/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070420
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Nucleosomes)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - CHO Cells
MH  - Cricetinae
MH  - Cricetulus
MH  - DNA, Ribosomal/*genetics
MH  - Humans
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Microscopy, Atomic Force
MH  - Nucleosomes/*genetics/ultrastructure
MH  - Oligonucleotide Array Sequence Analysis
MH  - Promoter Regions, Genetic/genetics
EDAT- 2007/04/21 09:00
MHDA- 2007/06/15 09:00
CRDT- 2007/04/21 09:00
PHST- 2007/04/21 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2007/04/21 09:00 [entrez]
AID - 10.1021/bi062116z [doi]
PST - ppublish
SO  - Biochemistry. 2007 May 15;46(19):5623-34. doi: 10.1021/bi062116z. Epub 2007 Apr 
      20.