PMID- 174447
OWN - NLM
STAT- MEDLINE
DCOM- 19760401
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 229
IP  - 6
DP  - 1975 Dec
TI  - Accumulation of some secretory enzymes in venom glands of Vipera palaestinae.
PG  - 1675-9
AB  - Secretion of venom in the venom glands of Vipera palaestinae was studied by 
      measuring the protein content and enzymatic activities of L-amino acid oxidase 
      (LAO), phosphodiesterase (PDE), and benzoylarginine ethyl esterase (BAEE). These 
      were tested in the accumulating venom and gland homogenates at 0, 2, 3, 4,6, and 
      15 days after an intitial emptying of the venom glands by milking. Changes in the 
      total activities of the enzymes and in the protein concentration were found in 
      the venom samples, but not in the homogenates, at the different intervals after 
      milking. In the venom the total activities of LAO, PDE, and BAEE were higher the 
      longer the time intervals from the initial milking. When the data were fit by a 
      straight line, the fluctuations from the line were of opposite signs for LAO and 
      PDE at the 3- and the 4-day intervals. There were no significant correlations 
      between the specific activities or between the changes in the specific activities 
      of any two of the enzymes at any time interval. It is concluded that each of the 
      enzymes is secreted at a rate independent of the other two; this pattern of 
      secretion can best be described as nonparallel.
FAU - Brown, R S
AU  - Brown RS
FAU - Brown, M B
AU  - Brown MB
FAU - Bdolah, A
AU  - Bdolah A
FAU - Kochva, E
AU  - Kochva E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Snake Venoms)
RN  - EC 1.4.- (Amino Acid Oxidoreductases)
RN  - EC 3.1.- (Esterases)
RN  - EC 3.1.4.- (Phosphoric Diester Hydrolases)
SB  - IM
MH  - Amino Acid Oxidoreductases/*analysis
MH  - Animals
MH  - Electrophoresis
MH  - Esterases/*analysis
MH  - Exocrine Glands/analysis/*enzymology
MH  - Fasting
MH  - Phosphoric Diester Hydrolases/*analysis
MH  - *Snake Venoms
MH  - Snakes/*metabolism
EDAT- 1975/12/01 00:00
MHDA- 1975/12/01 00:01
CRDT- 1975/12/01 00:00
PHST- 1975/12/01 00:00 [pubmed]
PHST- 1975/12/01 00:01 [medline]
PHST- 1975/12/01 00:00 [entrez]
AID - 10.1152/ajplegacy.1975.229.6.1675 [doi]
PST - ppublish
SO  - Am J Physiol. 1975 Dec;229(6):1675-9. doi: 10.1152/ajplegacy.1975.229.6.1675.