PMID- 17445167
OWN - NLM
STAT- MEDLINE
DCOM- 20070621
LR  - 20111117
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 69 Suppl 1
DP  - 2007 Apr
TI  - HLA-DRB sequencing-based typing: an improved protocol which shows complete DRB 
      exon 2 sequences and includes exon 3 of HLA-DRB4/5.
PG  - 61-3
AB  - Several human leukocyte antigen (HLA)-DRB protocols for sequencing-based typing 
      have been described. In general, the DRB1 amplification is performed using 
      group-specific amplification primers (GSAPs) located in HVR I or intron 1. Only 
      some protocols include amplification of DRB3, DRB4, and DRB5. However, prior 
      knowledge obtained by alternative methods such as PCR-SSP is preferred for some 
      protocols and a large amount of DNA is often required for adequate typing of 
      HLA-DRB1. We describe a new protocol that uses GSAPs located in the introns, 
      includes also sequencing of exon 3 to enable high resolution. This protocol 
      allows DRB typing without prior knowledge of the HLA type.
FAU - van Dijk, A
AU  - van Dijk A
AD  - Department of Pathology, University Medical Centre Utrecht, Utrecht, The 
      Netherlands.
FAU - Melchers, R
AU  - Melchers R
FAU - Hilkes, Y
AU  - Hilkes Y
FAU - Rozemuller, E
AU  - Rozemuller E
FAU - Tilanus, M
AU  - Tilanus M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (DNA Primers)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (HLA-DRB4 Chains)
RN  - 0 (HLA-DRB5 Chains)
SB  - IM
MH  - DNA Primers
MH  - Exons/*genetics
MH  - HLA-DR Antigens/*genetics
MH  - HLA-DRB4 Chains
MH  - HLA-DRB5 Chains
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Introns/genetics
MH  - Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
EDAT- 2007/04/21 09:00
MHDA- 2007/06/22 09:00
CRDT- 2007/04/21 09:00
PHST- 2007/04/21 09:00 [pubmed]
PHST- 2007/06/22 09:00 [medline]
PHST- 2007/04/21 09:00 [entrez]
AID - TAN760_4 [pii]
AID - 10.1111/j.1399-0039.2006.760_4.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2007 Apr;69 Suppl 1:61-3. doi: 10.1111/j.1399-0039.2006.760_4.x.