PMID- 17445184
OWN - NLM
STAT- MEDLINE
DCOM- 20070621
LR  - 20161025
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 69 Suppl 1
DP  - 2007 Apr
TI  - Killer cell immunoglobulin-like receptor polymorphisms in HLA-identical kidney 
      transplant recipients: lack of 2DL2 and 2DS2 may be associated with poor graft 
      function.
PG  - 123-4
AB  - Killer cell immunoglobulin-like receptors (KIR) on natural killer cells mediate 
      killing by recognizing class I presentation of peptides by infected or oncogenic 
      cells. KIR differences in stem cell transplants have been implicated in increased 
      graft vs host disease. Human leukocyte antigen (HLA)-matched-related kidney 
      recipients have the best graft survival as compared to one haplotype-matched 
      recipients. These HLA-identical transplant pairs may be ideal for studying minor 
      HLA antigens and KIR polymorphic differences and their relation to graft 
      function. We have studied KIR polymorphism in recipients and donor pairs of 
      HLA-matched sibling kidney transplants to demonstrate differences in genotype as 
      related to long-term graft function and/or chronic rejection. We employed a KIR 
      genotyping kit (Dynal, Brown Deer, WI), that uses sequence-specific priming by 
      PCR to identify 19 alleles for genotypes in 12 donor/recipient (D/R) pairs at 
      least 1 year posttransplant. There were two pairs that had different alleles in 
      the recipient that were not found in the donor. One pair had different alleles 
      found in the donor that were not present in the recipient. Two pairs had 
      difference in alleles in both the donor and recipient. Seven of the 12 pairs had 
      the same KIR genotype. Eight of the 12 pairs (both donor and recipient) exhibited 
      a haplotype with 2DL2+ and 2DS2+. Four of the 12 exhibited a haplotype 2DL2- and 
      2DS2-. Three out of four of these recipients had increased creatinine levels and 
      at least one graded rejection episode. One of these three has lost their graft. 
      In conclusion, the genotyping of HLA-matched sibling kidney transplant D/R pairs 
      demonstrates that there may be an association of higher risk for poor graft 
      function when both genotypes lack 2DL2 and 2DS2.
FAU - Cirocco, R E
AU  - Cirocco RE
AD  - The Lillian Jean Kaplan Renal Transplant Center of the Division of 
      Transplantation of the Department of Surgery at the University of Miami, Leonard 
      M. Miller School of Medicine, Miami, FL 33136, USA. rcirocco@med.miami.edu
FAU - Mathew, J M
AU  - Mathew JM
FAU - Burke, G W 3rd
AU  - Burke GW 3rd
FAU - Esquenazi, V
AU  - Esquenazi V
FAU - Miller, J
AU  - Miller J
LA  - eng
GR  - R01 DK025243/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA Antigens)
RN  - 0 (KIR2DS2 protein, human)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, KIR)
SB  - IM
MH  - Genotype
MH  - Graft Survival/*genetics
MH  - Graft vs Host Disease/*etiology
MH  - HLA Antigens/*genetics/metabolism
MH  - Hematopoietic Stem Cell Transplantation/*methods/mortality
MH  - Histocompatibility Testing
MH  - Humans
MH  - Killer Cells, Natural/immunology
MH  - Neoplasm Recurrence, Local/genetics
MH  - Receptors, Immunologic/deficiency/*genetics/immunology
MH  - Receptors, KIR
MH  - Siblings
EDAT- 2007/04/21 09:00
MHDA- 2007/06/22 09:00
CRDT- 2007/04/21 09:00
PHST- 2007/04/21 09:00 [pubmed]
PHST- 2007/06/22 09:00 [medline]
PHST- 2007/04/21 09:00 [entrez]
AID - TAN76211 [pii]
AID - 10.1111/j.1399-0039.2006.76211.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2007 Apr;69 Suppl 1:123-4. doi: 
      10.1111/j.1399-0039.2006.76211.x.