PMID- 17445192
OWN - NLM
STAT- MEDLINE
DCOM- 20070621
LR  - 20111117
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 69 Suppl 1
DP  - 2007 Apr
TI  - Recurrent spontaneous abortions patients have more -14 bp/+14 bp heterozygotes in 
      the 3'UT region of the HLA-G gene in a Chinese Han population.
PG  - 153-5
AB  - Although human leukocyte antigen (HLA)-G gene polymorphism is low, it may 
      influence levels of protein expression and, in some cases, has been associated 
      with pregnancy diseases. Apart from the 23 HLA-G alleles, including a null allele 
      known as HLA-G*0105N, a 14-bp deletion or insertion in the 3'UT region also 
      contributes to the HLA-G gene polymorphism. In order to discover any correlation 
      between the 14-bp deletion polymorphism and pregnancy diseases, we isolated the 
      genomic DNA from 24 recurrent spontaneous abortions (RSA) patients and 88 normal 
      fertile control individuals using the salted out method. Exon-8 of HLA-G gene of 
      the two groups was amplified by polymerase chain reaction, respectively, and 
      analyzed by electrophoresis on 9% nondenaturing polyacrylamide gel and stained 
      with ethidium bromide. Differences between the two groups were compared using the 
      chi(2) test. The results were there was no significant difference in allelic 
      frequencies of 14-bp deletion polymorphism between normal control and RSA 
      patients; frequency of -14 bp/+14 bp heterozygotes was significantly increased in 
      RSA patients as compared with normal fertile controls. Our results are similar to 
      a study of Indian women but contradictory to a study of Danish women. This 
      discrepancy may be due to ethnic differences.
FAU - Xue, S
AU  - Xue S
AD  - Shanghai Jiao Tong University School of Medicine, Shanghai Institute of 
      Immunology, Shanghai, 200025 China.
FAU - Yang, J
AU  - Yang J
FAU - Yao, F
AU  - Yao F
FAU - Xu, L
AU  - Xu L
FAU - Fan, L
AU  - Fan L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - 3' Untranslated Regions/*genetics
MH  - Abortion, Habitual/*genetics/immunology
MH  - Abortion, Spontaneous/*genetics/immunology
MH  - Base Sequence
MH  - China
MH  - Female
MH  - Gene Frequency
MH  - HLA Antigens/*genetics/immunology
MH  - HLA-G Antigens
MH  - Heterozygote
MH  - Histocompatibility Antigens Class I/*genetics/immunology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Pregnancy
EDAT- 2007/04/21 09:00
MHDA- 2007/06/22 09:00
CRDT- 2007/04/21 09:00
PHST- 2007/04/21 09:00 [pubmed]
PHST- 2007/06/22 09:00 [medline]
PHST- 2007/04/21 09:00 [entrez]
AID - TAN763_7 [pii]
AID - 10.1111/j.1399-0039.2006.763_7.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2007 Apr;69 Suppl 1:153-5. doi: 
      10.1111/j.1399-0039.2006.763_7.x.