PMID- 17445214
OWN - NLM
STAT- MEDLINE
DCOM- 20070621
LR  - 20070420
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 69 Suppl 1
DP  - 2007 Apr
TI  - Fourier transform infrared imaging as a method for detection of HLA class I 
      expression in melanoma without the use of antibody.
PG  - 252-8
AB  - Human leukocyte antigen (HLA) class I expression in melanoma is usually assessed 
      using immunohistochemical staining. Here we report on the use of Fourier 
      transform infrared (FTIR) hyperspectral imaging, a method widely used in 
      two-dimensional analysis of chemical components, to study HLA class I expression 
      in tissue. Two-dimensional cluster colour images derived from unsupervised 
      hierarchical cluster analysis of FTIR hyperspectral data on melanoma sections 
      were compared with consecutive sections that were immunohistochemically stained 
      for class I expression. HLA-class-I-positive and -negative areas were 
      differentiated by FTIR cluster images in all eight melanoma sections investigated 
      without the need for antibody attachment. FTIR imaging enables the distinction of 
      HLA-class-I-positive from class-I-negative areas in melanoma. This method is 
      accurate, rapid and cost-effective.
FAU - Chew, S F
AU  - Chew SF
AD  - Centre for Biospectroscopy and School of Chemistry, Monash University, Victoria 
      3800, Australia.
FAU - Wood, B R
AU  - Wood BR
FAU - Kanaan, C
AU  - Kanaan C
FAU - Browning, J
AU  - Browning J
FAU - MacGregor, D
AU  - MacGregor D
FAU - Davis, I D
AU  - Davis ID
FAU - Cebon, J
AU  - Cebon J
FAU - Tait, B D
AU  - Tait BD
FAU - McNaughton, D
AU  - McNaughton D
LA  - eng
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Histocompatibility Antigens Class I/*metabolism
MH  - Humans
MH  - Melanoma/*diagnosis/metabolism
MH  - Skin Neoplasms/*diagnosis/metabolism
MH  - *Spectroscopy, Fourier Transform Infrared
EDAT- 2007/04/21 09:00
MHDA- 2007/06/22 09:00
CRDT- 2007/04/21 09:00
PHST- 2007/04/21 09:00 [pubmed]
PHST- 2007/06/22 09:00 [medline]
PHST- 2007/04/21 09:00 [entrez]
AID - TAN775 [pii]
AID - 10.1111/j.1399-0039.2006.00775.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2007 Apr;69 Suppl 1:252-8. doi: 
      10.1111/j.1399-0039.2006.00775.x.