PMID- 17445242
OWN - NLM
STAT- MEDLINE
DCOM- 20070712
LR  - 20220310
IS  - 0953-816X (Print)
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 25
IP  - 8
DP  - 2007 Apr
TI  - Prenatal choline availability modulates hippocampal neurogenesis and neurogenic 
      responses to enriching experiences in adult female rats.
PG  - 2473-82
AB  - Increased dietary intake of choline early in life improves performance of adult 
      rats on memory tasks and prevents their age-related memory decline. Because 
      neurogenesis in the adult hippocampus also declines with age, we investigated 
      whether prenatal choline availability affects hippocampal neurogenesis in adult 
      Sprague-Dawley rats and modifies their neurogenic response to environmental 
      stimulation. On embryonic days (ED) 12-17, pregnant rats ate a 
      choline-supplemented (SUP-5 g/kg), choline sufficient (SFF-1.1 g/kg), or 
      choline-free (DEF) semisynthetic diet. Adult offspring either remained in 
      standard housing or were given 21 daily visits to explore a maze. On the last ten 
      exploration days, all rats received daily injections of 5-bromo-2-deoxyuridine 
      (BrdU, 100 mg/kg). The number of BrdU+ cells was significantly greater in the 
      dentate gyrus in SUP rats compared to SFF or DEF rats. While maze experience 
      increased the number of BrdU+ cells in SFF rats to the level seen in the SUP 
      rats, this enriching experience did not alter cell proliferation in DEF rats. 
      Similar patterns of cell proliferation were obtained with immunohistochemical 
      staining for neuronal marker doublecortin, confirming that diet and exploration 
      affected hippocampal neurogenesis. Moreover, hippocampal levels of the 
      brain-derived neurotrophic factor (BDNF) were increased in SUP rats as compared 
      to SFF and DEF animals. We conclude that prenatal choline intake has enduring 
      effects on adult hippocampal neurogenesis, possibly via up-regulation of BDNF 
      levels, and suggest that these alterations of neurogenesis may contribute to the 
      mechanism of life-long changes in cognitive function governed by the availability 
      of choline during gestation.
FAU - Glenn, Melissa J
AU  - Glenn MJ
AD  - Department of Psychology and Neuroscience, 572 Research Drive, Duke University, 
      Durham, NC 27708, USA. melissa.glenn@duke.edu
FAU - Gibson, Erin M
AU  - Gibson EM
FAU - Kirby, Elizabeth D
AU  - Kirby ED
FAU - Mellott, Tiffany J
AU  - Mellott TJ
FAU - Blusztajn, Jan K
AU  - Blusztajn JK
FAU - Williams, Christina L
AU  - Williams CL
LA  - eng
GR  - P01 AG009525-160004/AG/NIA NIH HHS/United States
GR  - F32 AG025052/AG/NIA NIH HHS/United States
GR  - P01 AG009525-140004/AG/NIA NIH HHS/United States
GR  - P01 AG009525-150004/AG/NIA NIH HHS/United States
GR  - P01 AG009525-13A10004/AG/NIA NIH HHS/United States
GR  - P01 AG009525/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Dcx protein, rat)
RN  - 0 (Doublecortin Protein)
RN  - G34N38R2N1 (Bromodeoxyuridine)
RN  - N91BDP6H0X (Choline)
SB  - IM
MH  - Animals
MH  - Behavior, Animal/physiology
MH  - Bromodeoxyuridine/metabolism
MH  - Choline/administration & dosage/*metabolism
MH  - *Diet
MH  - Dietary Supplements
MH  - Doublecortin Protein
MH  - Exploratory Behavior/physiology
MH  - Female
MH  - Hippocampus/cytology/*growth & development/physiology
MH  - Male
MH  - Neurons/cytology/*physiology
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC2435208
MID - NIHMS53609
EDAT- 2007/04/21 09:00
MHDA- 2007/07/13 09:00
PMCR- 2008/06/21
CRDT- 2007/04/21 09:00
PHST- 2007/04/21 09:00 [pubmed]
PHST- 2007/07/13 09:00 [medline]
PHST- 2007/04/21 09:00 [entrez]
PHST- 2008/06/21 00:00 [pmc-release]
AID - EJN5505 [pii]
AID - 10.1111/j.1460-9568.2007.05505.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2007 Apr;25(8):2473-82. doi: 10.1111/j.1460-9568.2007.05505.x.