PMID- 17445563
OWN - NLM
STAT- MEDLINE
DCOM- 20070719
LR  - 20171116
IS  - 0041-1345 (Print)
IS  - 0041-1345 (Linking)
VI  - 39
IP  - 3
DP  - 2007 Apr
TI  - Generation of dendritic cells with regulatory properties.
PG  - 633-7
AB  - Dendritic cells (DCs) are professional antigen presenting cells with the ability 
      to induce and regulate an immune response. DCs that capture and present antigen 
      under noninflammatory conditions maintain an immature phenotype and acquire 
      tolerogenic properties. These DCs generate regulatory T lymphocytes that 
      potentiate tolerogenic responses. Here we developed a method for the generation 
      of immature murine DCs able to process and present a specific antigen in a 
      tolerogenic context. Immature DCs were prepared from bone marrow precursors after 
      differentiation with granulocyte-macrophage colony-stimulating factor (GM-CSF) in 
      the presence of vitamin D(3) and characterized by their low expression of major 
      histocompatibility complex class (MHC) II and CD86 molecules. Purified phagosomes 
      containing either MHC II molecules or ovalbumin were used to deliver antigens to 
      immature DCs. More than 80% of the DCs captured the phagosomes, while maintaining 
      a low expression of maturation markers and showing basal levels of secretion of 
      activating cytokines such as interleukin (IL)-2 and IL-12. Treatment of the 
      immature DCs with lipopolysaccharides (LPS) increased IL-10 secretion, in 
      agreement with their anti-inflammatory and immune regulatory properties. 
      Cocultures of transgenic OT-II T lymphocytes with the immature DCs carrying 
      OVA-phagosomes succeeded in generating a subpopulation of regulatory T 
      lymphocytes characterized by the expression of CD4, CD25, CD62L, and Foxp3. Taken 
      together, our results suggest that vitamin D(3) generates immune tolerance 
      through the modulation of DC phenotype and could be useful to induce tolerance to 
      allotransplants.
FAU - Ureta, G
AU  - Ureta G
AD  - Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Santiago, 
      Chile.
FAU - Osorio, F
AU  - Osorio F
FAU - Morales, J
AU  - Morales J
FAU - Rosemblatt, M
AU  - Rosemblatt M
FAU - Bono, M R
AU  - Bono MR
FAU - Fierro, J A
AU  - Fierro JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (B7-2 Antigen)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 9006-59-1 (Ovalbumin)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Animals
MH  - B7-2 Antigen/drug effects
MH  - Bone Marrow Cells/immunology
MH  - Calcitriol/pharmacology
MH  - Cell Differentiation
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Histocompatibility Antigens Class II/drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Ovalbumin/genetics
MH  - Phagocytosis/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes, Regulatory/drug effects/*immunology
EDAT- 2007/04/21 09:00
MHDA- 2007/07/20 09:00
CRDT- 2007/04/21 09:00
PHST- 2007/04/21 09:00 [pubmed]
PHST- 2007/07/20 09:00 [medline]
PHST- 2007/04/21 09:00 [entrez]
AID - S0041-1345(06)01578-8 [pii]
AID - 10.1016/j.transproceed.2006.12.032 [doi]
PST - ppublish
SO  - Transplant Proc. 2007 Apr;39(3):633-7. doi: 10.1016/j.transproceed.2006.12.032.