PMID- 17446294
OWN - NLM
STAT- MEDLINE
DCOM- 20070607
LR  - 20200403
IS  - 0022-2615 (Print)
IS  - 0022-2615 (Linking)
VI  - 56
IP  - Pt 5
DP  - 2007 May
TI  - The in vivo dynamics of Streptococcus spp., Actinomyces naeslundii, Fusobacterium 
      nucleatum and Veillonella spp. in dental plaque biofilm as analysed by 
      five-colour multiplex fluorescence in situ hybridization.
PG  - 681-687
LID - 10.1099/jmm.0.47094-0 [doi]
AB  - The formation and composition of dental plaque biofilm in vivo are important 
      factors which influence the development of gingivitis, caries and periodontitis. 
      Studying dental plaque biofilm in in vitro models can cause an oversimplification 
      of the real conditions in the oral cavity. In this study, bovine enamel slabs 
      were fixed in an individual acrylic appliance in situ to quantify dental plaque 
      formation and composition using multiplex fluorescence in situ hybridization 
      (FISH) and confocal laser scanning microscopy. Each of the five oligonucleotide 
      probes used for FISH was specific for either eubacteria or one of four frequently 
      isolated bacterial constituents belonging to early and late colonizers of tooth 
      surfaces. The thickness of formed biofilm increased from 14.9+/-5.0 microm after 
      1 day to 49.3+/-11.6 microm after 7 days. Streptococcus spp. were predominant in 
      1-day-old dental plaque and decreased significantly after 7 days (P=0.0061). 
      Compared to the first day, Fusobacterium nucleatum decreased after 2 days and 
      increased significantly after 7 days (P=0.0006). The decreases of Actinomyces 
      naeslundii content on day 2 and day 7 were significant (P=0.0028). Changes in 
      Veillonella spp. were not significant during the study period (P >0.05). The 
      results showed that an in vivo observation period of 7 days was required to 
      detect significant changes in Streptococcus spp. and F. nucleatum. The multiplex 
      FISH used is suitable for analysing the dynamics of four important bacterial 
      constituents in the oral biofilm in epidemiological studies.
FAU - Al-Ahmad, Ali
AU  - Al-Ahmad A
AD  - Department of Operative Dentistry and Periodontology, Albert-Ludwigs-University, 
      Freiburg, Germany.
FAU - Wunder, Axel
AU  - Wunder A
AD  - Department of Operative Dentistry and Periodontology, Albert-Ludwigs-University, 
      Freiburg, Germany.
FAU - Auschill, Thorsten Mathias
AU  - Auschill TM
AD  - Department of Operative Dentistry and Periodontology, Albert-Ludwigs-University, 
      Freiburg, Germany.
FAU - Follo, Marie
AU  - Follo M
AD  - Department of Hematology and Oncology, Core Facility, Albert-Ludwigs-University, 
      Freiburg, Germany.
FAU - Braun, Gabriele
AU  - Braun G
AD  - Department of Operative Dentistry and Periodontology, Albert-Ludwigs-University, 
      Freiburg, Germany.
FAU - Hellwig, Elmar
AU  - Hellwig E
AD  - Department of Operative Dentistry and Periodontology, Albert-Ludwigs-University, 
      Freiburg, Germany.
FAU - Arweiler, Nicole Birgit
AU  - Arweiler NB
AD  - Department of Operative Dentistry and Periodontology, Albert-Ludwigs-University, 
      Freiburg, Germany.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
SB  - IM
MH  - Actinomyces/*growth & development
MH  - Adult
MH  - *Biofilms
MH  - Colony Count, Microbial
MH  - Dental Enamel/microbiology
MH  - Dental Plaque/*microbiology
MH  - Fusobacterium nucleatum/*growth & development
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy, Confocal
MH  - Streptococcus/*growth & development
MH  - Time Factors
MH  - Veillonella/*growth & development
EDAT- 2007/04/21 09:00
MHDA- 2007/06/08 09:00
CRDT- 2007/04/21 09:00
PHST- 2007/04/21 09:00 [pubmed]
PHST- 2007/06/08 09:00 [medline]
PHST- 2007/04/21 09:00 [entrez]
AID - 10.1099/jmm.0.47094-0 [doi]
PST - ppublish
SO  - J Med Microbiol. 2007 May;56(Pt 5):681-687. doi: 10.1099/jmm.0.47094-0.