PMID- 17447420
OWN - NLM
STAT- MEDLINE
DCOM- 20070517
LR  - 20191026
IS  - 0303-6995 (Print)
IS  - 0303-6995 (Linking)
IP  - 71
DP  - 2006
TI  - Isatin interaction with glyceraldehyde-3-phosphate dehydrogenase, a putative 
      target of neuroprotective drugs: partial agonism with deprenyl.
PG  - 97-103
AB  - There is evidence that the binding of deprenyl, a monoamine oxidase (MAO) B 
      inhibitor, and other propargylamines to glyceraldehyde-3-phosphate dehydrogenase 
      (GAPDH) is primarily responsible for their neuroprotective and antiapoptotic 
      effects. Thus, GAPDH may be a target for other neuroprotective drugs. Using two 
      independent approaches, radioligand analysis and an optical biosensor technique, 
      we demonstrate here that GAPDH also interacts with the endogenous, reversible MAO 
      B inhibitor, isatin. Deprenyl inhibited both [3H]isatin binding to GAPDH, and the 
      binding of this enzyme to an isatin analogue, 5-aminoisatin, immobilized on to an 
      optical biosensor cell. Another MAO inhibitor, tranylcypromine, was ineffective. 
      Both deprenyl and isatin inhibited GAPDH-mediated cleavage of E. coli tRNA, and 
      their effects were not additive. We suggest that isatin may be an endogenous 
      partial functional agonist of deprenyl in its effect on GAPDH and GAPDH-mediated 
      RNA cleavage. Changes in level of endogenous isatin may influence the 
      neuroprotective effect of deprenyl in vivo.
FAU - Medvedev, A
AU  - Medvedev A
AD  - Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow, 
      Russia. Alexei.Medvedev@ibmc.msk.ru
FAU - Buneeva, O
AU  - Buneeva O
FAU - Gnedenko, O
AU  - Gnedenko O
FAU - Fedchenko, V
AU  - Fedchenko V
FAU - Medvedeva, M
AU  - Medvedeva M
FAU - Ivanov, Y
AU  - Ivanov Y
FAU - Glover, V
AU  - Glover V
FAU - Sandler, M
AU  - Sandler M
LA  - eng
GR  - 072381/Z/03/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - J Neural Transm Suppl
JT  - Journal of neural transmission. Supplementum
JID - 0425126
RN  - 0 (Monoamine Oxidase Inhibitors)
RN  - 0U46U6E8UK (NAD)
RN  - 10028-17-8 (Tritium)
RN  - 2K1V7GP655 (Selegiline)
RN  - 82X95S7M06 (Isatin)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - Biosensing Techniques/methods
MH  - Dose-Response Relationship, Drug
MH  - Drug Interactions
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*metabolism
MH  - Isatin/*metabolism
MH  - Monoamine Oxidase Inhibitors/pharmacokinetics/*pharmacology
MH  - Muscles/chemistry
MH  - NAD/pharmacokinetics
MH  - Protein Array Analysis/methods
MH  - Protein Binding/drug effects
MH  - Rabbits
MH  - Selegiline/pharmacokinetics/*pharmacology
MH  - Time Factors
MH  - Tritium/metabolism
EDAT- 2007/04/24 09:00
MHDA- 2007/05/18 09:00
CRDT- 2007/04/24 09:00
PHST- 2007/04/24 09:00 [pubmed]
PHST- 2007/05/18 09:00 [medline]
PHST- 2007/04/24 09:00 [entrez]
AID - 10.1007/978-3-211-33328-0_11 [doi]
PST - ppublish
SO  - J Neural Transm Suppl. 2006;(71):97-103. doi: 10.1007/978-3-211-33328-0_11.