PMID- 17448455
OWN - NLM
STAT- MEDLINE
DCOM- 20070626
LR  - 20181201
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 244
IP  - 1
DP  - 2006 Nov
TI  - The immunobiological development of human bone marrow mesenchymal stem cells in 
      the course of neuronal differentiation.
PG  - 19-32
AB  - The central nervous system (CNS) has been referred to as the "immunological 
      privileged site". However, it is now clear that the privileged status of the CNS 
      is a result of a balance between immune privilege and effective response. In 
      vitro, human bone marrow mesenchymal stem cells (MSCs) have the ability to 
      differentiate into neurons. Based on this biological attribute we gain the 
      possibility by means of using MSCs as the donors to develop a future cell therapy 
      in clinical application. But using MSCs as donor cells inevitably raises the 
      question as to whether these donor cells would be immunogenic, and if so, would 
      they be rejected after transplantation. To investigate this, human MSCs were 
      cultured in vitro and induced to differentiate along neuronal lineage. The 
      expression of human leukocyte antigen (HLA) class I and class II molecules and 
      the co-stimulatory protein CD80 were increased on the surface of MSCs in the 
      course of neuronal differentiation. But neither of the co-stimulatory proteins, 
      CD40 or CD86, was expressed. After IFN-gamma exposure, the expression of the HLA 
      molecules was further enhanced, but the co-stimulatory proteins were unaffected. 
      MSCs that had been differentiated along neuronal lineage were not capable of 
      inducing the proliferation of peripheral blood lymphocytes (PBLs). Even after 
      IFN-gamma exposure, PBLs remained unresponsive. Furthermore, MSCs differentiated 
      along neuronal lineage suppressed the proliferation of PBLs induced by allogeneic 
      PBLs and mitogens. The mechanisms involved in the immunosuppression may be 
      related to the effect of soluble factors and cell-cell interactions of neuronal 
      differentiated MSCs and PBLs. From the above data we suggested that the low 
      immunogenicity and immunomodulatory function of MSCs in the course of neuronal 
      differentiation in vitro, which will be helpful to further investigation in order 
      to establish the new way for future medical application.
FAU - Liu, Chen-Tao
AU  - Liu CT
AD  - XiangYa Hospital, Central South University 87 Xiangya Road, Changsha, Hunan 
      410008, China.
FAU - Yang, Yu-Jia
AU  - Yang YJ
FAU - Yin, Fei
AU  - Yin F
FAU - Wang, Xia
AU  - Wang X
FAU - Yu, Xiao-He
AU  - Yu XH
FAU - Wang, Qing-Hong
AU  - Wang QH
FAU - Wang, Xiao-Li
AU  - Wang XL
FAU - Xie, Min
AU  - Xie M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070419
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (Histocompatibility Antigens)
SB  - IM
MH  - B7-2 Antigen/biosynthesis
MH  - Blotting, Western
MH  - Bone Marrow Cells
MH  - CD40 Antigens/biosynthesis
MH  - Cell Differentiation/*immunology
MH  - Cell Proliferation
MH  - Histocompatibility Antigens/biosynthesis
MH  - Humans
MH  - Lymphocyte Culture Test, Mixed
MH  - Lymphocytes/immunology
MH  - Mesenchymal Stem Cells/*cytology/*immunology
MH  - Neurons/*cytology/*immunology
EDAT- 2007/04/24 09:00
MHDA- 2007/06/27 09:00
CRDT- 2007/04/24 09:00
PHST- 2006/10/25 00:00 [received]
PHST- 2007/01/26 00:00 [revised]
PHST- 2007/02/07 00:00 [accepted]
PHST- 2007/04/24 09:00 [pubmed]
PHST- 2007/06/27 09:00 [medline]
PHST- 2007/04/24 09:00 [entrez]
AID - S0008-8749(07)00019-6 [pii]
AID - 10.1016/j.cellimm.2007.02.003 [doi]
PST - ppublish
SO  - Cell Immunol. 2006 Nov;244(1):19-32. doi: 10.1016/j.cellimm.2007.02.003. Epub 
      2007 Apr 19.