PMID- 17448548
OWN - NLM
STAT- MEDLINE
DCOM- 20071004
LR  - 20151119
IS  - 0166-445X (Print)
IS  - 0166-445X (Linking)
VI  - 83
IP  - 1
DP  - 2007 Jun 5
TI  - Effects of lead, molybdenum, rubidium, arsenic and organochlorines on 
      spermatogenesis in fish: monitoring at Mekong Delta area and in vitro experiment.
PG  - 43-51
AB  - To estimate the influence of water contaminants on fish reproduction in the 
      Mekong Delta area, we sampled cultivated male catfish (Pangasianodon 
      hypophthalmus), investigated testicular development, and measured persistent 
      organic pollutants (POPs) and trace element levels in muscle and liver, 
      respectively. Various testes sizes were observed although sampling took place 
      during a short period. Histological analysis revealed that all developmental 
      stages of germ cells were observed in catfish with large testis, whereas only 
      necrotic spermatogonia but no other germ cells were observed in catfish with 
      small testis. In small testis, furthermore, vacuolization and hypertrophy of 
      Sertoli cells were observed. Measurement of POPs in muscle and trace elements in 
      liver demonstrated that there were negative correlations between GSI and the 
      concentrations of Pb, Mo, Rb and As. To clarify possible direct effects of Pb, 
      Mo, Rb and As on spermatogenesis in fish, we investigated the effects of these 
      trace elements on spermatogenesis using in vitro testicular organ culture of 
      Japanese eel (Anguilla japonica). Treatment with each of the trace elements alone 
      did not affect spermatogenesis. However, treatment with 10(-7)M of Pb, 10(-5) and 
      10(-4)M of Mo, 10(-5)-10(-3)M of Rb or 10(-5)M of As inhibited the 
      spermatogenesis induced by 11-ketotestosterone (11KT). Furthermore, treatment 
      with 10(-4)M of As in combination with 11KT caused necrosis of testicular 
      fragments. Taken together, these results are consistent with the hypothesis that 
      Pb, Mo, Rb and As can exert inhibitory effects on spermatogenesis in catfish 
      inhabiting the Mekong Delta area.
FAU - Yamaguchi, Sonoko
AU  - Yamaguchi S
AD  - Laboratory of Fish Reproductive Physiology, Ehime University, Matsuyama, Ehime 
      790-8566, Japan.
FAU - Miura, Chiemi
AU  - Miura C
FAU - Ito, Aki
AU  - Ito A
FAU - Agusa, Tetsuro
AU  - Agusa T
FAU - Iwata, Hisato
AU  - Iwata H
FAU - Tanabe, Shinsuke
AU  - Tanabe S
FAU - Tuyen, Bui Cach
AU  - Tuyen BC
FAU - Miura, Takeshi
AU  - Miura T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070318
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Metals, Heavy)
RN  - 0 (Water Pollutants, Chemical)
RN  - 3XMK78S47O (Testosterone)
RN  - KF38W1A85U (11-ketotestosterone)
RN  - N712M78A8G (Arsenic)
SB  - IM
MH  - Anguilla/*physiology
MH  - Animals
MH  - Arsenic/*toxicity
MH  - Catfishes/*physiology
MH  - Hydrocarbons, Chlorinated/analysis/*toxicity
MH  - Liver/chemistry
MH  - Male
MH  - Metals, Heavy/*toxicity
MH  - Muscles/chemistry
MH  - Organ Culture Techniques/veterinary
MH  - Spermatogenesis/*drug effects
MH  - Statistics as Topic
MH  - Testis/drug effects/pathology
MH  - Testosterone/analogs & derivatives/blood
MH  - Vietnam
MH  - Water Pollutants, Chemical/toxicity
EDAT- 2007/04/24 09:00
MHDA- 2007/10/05 09:00
CRDT- 2007/04/24 09:00
PHST- 2006/12/25 00:00 [received]
PHST- 2007/03/14 00:00 [revised]
PHST- 2007/03/16 00:00 [accepted]
PHST- 2007/04/24 09:00 [pubmed]
PHST- 2007/10/05 09:00 [medline]
PHST- 2007/04/24 09:00 [entrez]
AID - S0166-445X(07)00110-5 [pii]
AID - 10.1016/j.aquatox.2007.03.010 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2007 Jun 5;83(1):43-51. doi: 10.1016/j.aquatox.2007.03.010. Epub 
      2007 Mar 18.