PMID- 17451817
OWN - NLM
STAT- MEDLINE
DCOM- 20071107
LR  - 20070618
IS  - 0166-3542 (Print)
IS  - 0166-3542 (Linking)
VI  - 75
IP  - 3
DP  - 2007 Sep
TI  - Enhanced protective efficacy of H5 subtype avian influenza DNA vaccine with codon 
      optimized HA gene in a pCAGGS plasmid vector.
PG  - 234-41
AB  - H5N1 influenza viruses have caused significant disease and deaths in various 
      parts of the world in several species, including humans. Vaccination combined 
      with culling can provide an attractive method for outbreak containment. Using 
      synthesized oligos and overlapping extension PCR techniques, we constructed an H5 
      HA gene, optiHA, containing chicken biased codons based on the HA amino acid 
      sequence of the highly pathogenic H5N1 virus A/goose/Guangdong/1/96 (GS/GD/96). 
      The optiHA and wild-type HA genes were inserted into plasmids pCI or pCAGGS, and 
      designated as pCIoptiHA, pCAGGoptiHA, pCIHA and pCAGGHA, respectively. To 
      evaluate vaccine efficacy, groups of 3-week-old specific pathogen free (SPF) 
      chickens were intramuscularly injected with the four plasmids. Sera were 
      collected on a weekly basis post-vaccination (p.v.) for hemagglutination 
      inhibition (HI) assays and neutralization (NT) antibody detection. All chickens 
      receiving pCAGGoptiHA and pCAGGHA developed high levels of HI and NT antibodies 
      at 3 weeks p.v., and were completely protected from lethal H5 virus challenge, 
      while only partial protection was induced by inoculation with the other two 
      plasmids. A second experiment was conducted to evaluate if a lower dose of the 
      pCAGGoptiHA vaccine could be effective, results indicated that two doses of 10 
      microg of pCAGGoptiHA could induce complete protection in chickens against H5 
      lethal virus challenge. Based on our results, we conclude that construction 
      optimization could dramatically increase the H5 HA gene DNA vaccine efficacy in 
      chickens, and therefore, greatly decrease the dose necessary for inducing 
      complete protection in chickens.
FAU - Jiang, Yongping
AU  - Jiang Y
AD  - Animal Influenza Laboratory of the Ministry of Agriculture, Harbin Veterinary 
      Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, 
      Harbin 150001, People's Republic of China.
FAU - Yu, Kangzhen
AU  - Yu K
FAU - Zhang, Hongbo
AU  - Zhang H
FAU - Zhang, Pingjing
AU  - Zhang P
FAU - Li, Chenjun
AU  - Li C
FAU - Tian, Guobin
AU  - Tian G
FAU - Li, Yanbing
AU  - Li Y
FAU - Wang, Xijun
AU  - Wang X
FAU - Ge, Jinying
AU  - Ge J
FAU - Bu, Zhigao
AU  - Bu Z
FAU - Chen, Hualan
AU  - Chen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070409
PL  - Netherlands
TA  - Antiviral Res
JT  - Antiviral research
JID - 8109699
RN  - 0 (Antibodies, Viral)
RN  - 0 (Hemagglutinin Glycoproteins, Influenza Virus)
RN  - 0 (Influenza Vaccines)
RN  - 0 (Vaccines, DNA)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - *Chickens/immunology/virology
MH  - Genetic Vectors
MH  - Hemagglutination Inhibition Tests
MH  - Hemagglutinin Glycoproteins, Influenza Virus/genetics/immunology
MH  - Influenza A Virus, H5N1 Subtype/genetics/*immunology/pathogenicity/physiology
MH  - Influenza Vaccines/*administration & dosage/genetics/immunology
MH  - Influenza in Birds/*prevention & control/virology
MH  - Neutralization Tests
MH  - Plasmids
MH  - Vaccines, DNA/*administration & dosage/genetics/immunology
MH  - Virus Shedding
EDAT- 2007/04/25 09:00
MHDA- 2007/11/08 09:00
CRDT- 2007/04/25 09:00
PHST- 2006/10/12 00:00 [received]
PHST- 2007/01/24 00:00 [revised]
PHST- 2007/03/16 00:00 [accepted]
PHST- 2007/04/25 09:00 [pubmed]
PHST- 2007/11/08 09:00 [medline]
PHST- 2007/04/25 09:00 [entrez]
AID - S0166-3542(07)00277-X [pii]
AID - 10.1016/j.antiviral.2007.03.009 [doi]
PST - ppublish
SO  - Antiviral Res. 2007 Sep;75(3):234-41. doi: 10.1016/j.antiviral.2007.03.009. Epub 
      2007 Apr 9.