PMID- 17451892
OWN - NLM
STAT- MEDLINE
DCOM- 20080708
LR  - 20080408
IS  - 0378-4320 (Print)
IS  - 0378-4320 (Linking)
VI  - 106
IP  - 1-2
DP  - 2008 Jun
TI  - The quantification of lipid and protein oxidation in stallion spermatozoa and 
      seminal plasma: seasonal distinctions and correlations with DNA strand breaks, 
      classical seminal parameters and stallion fertility.
PG  - 36-47
AB  - The goal of this work was to correlate oxidative stress caused by reactive oxygen 
      species (ROS) and DNA damage with classic semen parameters in spermatozoa and 
      seminal plasma of fertile and subfertile stallions. Oxidation was measured in 
      both lipids and proteins, using the thiobarbituric acid reactive species (TBARS) 
      assay and the DNPH carbonyl groups assay, respectively. Sperm DNA damage was 
      monitored using the TUNEL assay. These parameters were monitored in samples 
      obtained during the breeding and the non-breeding seasons. In general, fertile 
      stallions showed better classical semen parameters, and those parameters improved 
      from the non-breeding to the breeding season, although an increase in sperm 
      production was accompanied by a decrease in the semen quality from subfertile 
      stallions in the breeding season. In terms of oxidation levels we found that 
      there were clear differences whether lipids or proteins were considered. In the 
      breeding season there seemed to be a tendency towards normalizing lipid oxidation 
      in spermatozoa and seminal plasma, and protein oxidation in the seminal plasma, 
      of both fertile and subfertile animals. Thus, differences monitored in the 
      non-breeding season were no longer visible. Interestingly, a higher level of 
      protein oxidation was found in the sperm of fertile animals in the breeding 
      season. Considering that there were positive correlations between sperm protein 
      oxidation and sperm motility and vitality, these results suggests that the 
      oxidation of semen proteins may be important for sperm function. On the other 
      hand, lipid oxidation in the seminal plasma seemed to be a general indicator for 
      sperm damage. In the non-breeding season positive correlations between lipid and 
      protein oxidation levels in both sperm and seminal plasma and several defects in 
      sperm function were found, but only for subfertile animals, thus suggesting that 
      lipid and protein oxidation may aid in the identification of subfertile stallions 
      during the non-breeding season. Levels of ROS production never seemed to result 
      in compromised sperm DNA integrity, indicating that measurements were within 
      physiological levels and/or that there is an efficient antioxidant activity in 
      stallion sperm cells.
FAU - Morte, Maria Ines
AU  - Morte MI
AD  - Department of Zoology, Center for Neurocience and Cell Biology of Coimbra, 
      University of Coimbra, 3004-517 Coimbra, Portugal.
FAU - Rodrigues, Ana Margarida
AU  - Rodrigues AM
FAU - Soares, Diana
AU  - Soares D
FAU - Rodrigues, Ana Sofia
AU  - Rodrigues AS
FAU - Gamboa, Sandra
AU  - Gamboa S
FAU - Ramalho-Santos, Joao
AU  - Ramalho-Santos J
LA  - eng
PT  - Journal Article
DEP - 20070330
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 0 (Lipids)
RN  - 0 (Proteins)
SB  - IM
MH  - Acrosome/physiology
MH  - Animals
MH  - Breeding
MH  - *DNA Breaks, Double-Stranded
MH  - Fertility/genetics/*physiology
MH  - Horses/genetics/*physiology
MH  - Lipids/*analysis
MH  - Male
MH  - Oxidation-Reduction
MH  - Proteins/analysis/*metabolism
MH  - *Seasons
MH  - Semen/*chemistry/metabolism
MH  - Spermatozoa/*chemistry/metabolism
EDAT- 2007/04/25 09:00
MHDA- 2008/07/09 09:00
CRDT- 2007/04/25 09:00
PHST- 2006/11/21 00:00 [received]
PHST- 2007/03/06 00:00 [revised]
PHST- 2007/03/23 00:00 [accepted]
PHST- 2007/04/25 09:00 [pubmed]
PHST- 2008/07/09 09:00 [medline]
PHST- 2007/04/25 09:00 [entrez]
AID - S0378-4320(07)00131-5 [pii]
AID - 10.1016/j.anireprosci.2007.03.020 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2008 Jun;106(1-2):36-47. doi: 10.1016/j.anireprosci.2007.03.020. 
      Epub 2007 Mar 30.