PMID- 17452253
OWN - NLM
STAT- MEDLINE
DCOM- 20070611
LR  - 20131121
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 174
IP  - 2
DP  - 2007 Apr 15
TI  - "Home-brew" FISH assay shows higher efficiency than BCR-ABL dual color, dual 
      fusion probe in detecting microdeletions and complex rearrangements associated 
      with t(9;22) in chronic myeloid leukemia.
PG  - 121-6
AB  - We carried out fluorescence in situ hybridization (FISH) studies on 18 Ph+ 
      chronic myeloid leukemia (CML) cases with chromosome 22 genomic deletions with 
      the Vysis BCR-ABL dual-color/dual-fusion probe (BCR-ABL DC/DF) to compare the 
      hybridization patterns obtained with this approach to those obtained with the 
      "home brew" BAC/PAC system. Our results are the following: chromosome 22 
      microdeletions less than 400 kilobases (Kb) were not detected by the BCR DC/DF 
      probe; FISH analysis with the BCR DC/DF probe in cases bearing chromosome 22 
      microdeletions ranging from 400 to 700 Kb produced a faint signal on the der(9); 
      and the BCR-ABL DC/DF FISH pattern was comparable to the one obtained by the home 
      brew probe in the presence of a 900-Kb chromosome 22 microdeletion. Our home-brew 
      FISH system represents an accurate method for revealing a subset of CML patients 
      with der(9) microdeletions.
FAU - Albano, Francesco
AU  - Albano F
AD  - Department of Hematology, University of Bari, Policlinico, Piazza G. Cesare 11, 
      70124 Bari, Italy.
FAU - Anelli, Luisa
AU  - Anelli L
FAU - Zagaria, Antonella
AU  - Zagaria A
FAU - Archidiacono, Nicoletta
AU  - Archidiacono N
FAU - Liso, Vincenzo
AU  - Liso V
FAU - Specchia, Giorgina
AU  - Specchia G
FAU - Rocchi, Mariano
AU  - Rocchi M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Chromosome Deletion
MH  - *Chromosomes, Human, Pair 22
MH  - *Chromosomes, Human, Pair 9
MH  - DNA Probes/genetics
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - Reproducibility of Results
MH  - *Translocation, Genetic
EDAT- 2007/04/25 09:00
MHDA- 2007/06/15 09:00
CRDT- 2007/04/25 09:00
PHST- 2006/09/11 00:00 [received]
PHST- 2006/09/22 00:00 [revised]
PHST- 2006/09/29 00:00 [accepted]
PHST- 2007/04/25 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2007/04/25 09:00 [entrez]
AID - S0165-4608(06)00793-X [pii]
AID - 10.1016/j.cancergencyto.2006.09.025 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2007 Apr 15;174(2):121-6. doi: 
      10.1016/j.cancergencyto.2006.09.025.