PMID- 17455195
OWN - NLM
STAT- MEDLINE
DCOM- 20080226
LR  - 20071025
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 102
IP  - 4
DP  - 2007 Nov 1
TI  - A proximal direct repeat motif characterized as a negative regulatory element in 
      the human renin gene.
PG  - 1043-50
AB  - The regulation of renin gene expression is thought to be fundamental to 
      regulation of the total renin-angiotensin system. The human renin gene contains a 
      direct repeat (DR) motif AGGGGTCAC-AGGGCCA in the proximal region (-259/-245 bp), 
      which contains similar sequence for nuclear receptor superfamily binding core 
      motif, AGGTCA, and is the most similar to COUP-TFII consensus. The DR motif was 
      evaluated as a functional cis-element with renal cortex and chorio-decidual cells 
      by footprint assay, electromobility shift assay (EMSA) and reporter assay. The DR 
      motif site was protected by footprint analysis with a clear hypersensitive and a 
      minor hypersensitive region in good accordance with the DR of the consensus. One 
      of the binding proteins was strongly suspected to be COUP-TFII-consensus-specific 
      by EMSA. The DNA/protein complexes obtained with nuclear extract of renin 
      producing cells could be completely blocked by homologous competitor and strongly 
      blocked by the second-half mutant oligonucleotide of the DR motif but not by the 
      first-half mutant oligonucleotide. Finally, the transcriptional activity of 
      second-half mutant construct is slightly elevated and that first-half mutant 
      construct is significantly stronger by twofold compared with wild type construct 
      in reporter assay. These findings suggest that the DR motif site of the human 
      renin gene functions as a negative regulatory element involved in a twofold 
      repression of transcription and that member(s) of nucleic receptor superfamily 
      bind the site and play important roles in the human renin gene expression with a 
      possibility that one of the binding protein is COUP-TFII.
CI  - (c) 2007 Wiley-Liss, Inc.
FAU - Konoshita, Tadashi
AU  - Konoshita T
AD  - Third Department of Internal Medicine, Fukui University School of Medicine, 23-3, 
      Shimoaizuki, Matsuoka, 910-1193, Japan. konosita@fmsrsa.fukui-med.ac.jp
FAU - Fuchs, Sebastien
AU  - Fuchs S
FAU - Makino, Yasukazu
AU  - Makino Y
FAU - Wakahara, Shigeyuki
AU  - Wakahara S
FAU - Miyamori, Isamu
AU  - Miyamori I
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (COUP Transcription Factor II)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - EC 3.4.23.15 (Renin)
SB  - IM
MH  - Amino Acid Motifs
MH  - Base Sequence
MH  - Binding Sites
MH  - COUP Transcription Factor II/*metabolism
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Mutation
MH  - Protein Binding
MH  - Receptors, Cytoplasmic and Nuclear
MH  - Regulatory Elements, Transcriptional/*genetics
MH  - Renin/*genetics
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 2007/04/25 09:00
MHDA- 2008/02/27 09:00
CRDT- 2007/04/25 09:00
PHST- 2007/04/25 09:00 [pubmed]
PHST- 2008/02/27 09:00 [medline]
PHST- 2007/04/25 09:00 [entrez]
AID - 10.1002/jcb.21341 [doi]
PST - ppublish
SO  - J Cell Biochem. 2007 Nov 1;102(4):1043-50. doi: 10.1002/jcb.21341.