PMID- 17456576
OWN - NLM
STAT- MEDLINE
DCOM- 20070828
LR  - 20151119
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 92
IP  - 7
DP  - 2007 Jul
TI  - Monocyte chemoattractant protein-1 in subcutaneous abdominal adipose tissue: 
      characterization of interstitial concentration and regulation of gene expression 
      by insulin.
PG  - 2688-95
AB  - CONTEXT: The chemokine monocyte chemoattractant protein-1 (MCP-1) is implicated 
      in obesity-associated chronic inflammation, insulin resistance, and 
      atherosclerosis. OBJECTIVES: The objectives of this study were to: 1) 
      characterize the interstitial levels and the gene expression of MCP-1 in the sc 
      abdominal adipose tissue (SCAAT), 2) elucidate the response of MCP-1 to acute 
      hyperinsulinemia, and 3) determine the relationship between MCP-1 and arterial 
      stiffness. DESIGN: Nine lean (L) and nine uncomplicated obese (OB) males were 
      studied in the fasting state and during a euglycemic-hyperinsulinemic clamp 
      combined with the microdialysis technique. Interstitial and serum MCP-1 (iMCP-1 
      and sMCP-1, respectively) levels, pulse wave analysis, and SCAAT biopsies were 
      characterized at baseline and after hyperinsulinemia. RESULTS: OB showed elevated 
      sMCP-1 (P < 0.01) but similar iMCP-1 levels as compared with L. Basal iMCP-1 
      concentrations were considerably higher than sMCP-1 (P < 0.0001), and a gradient 
      between iMCP-1 and sMCP-1 levels was maintained throughout the hyperinsulinemia. 
      At baseline, SCAAT gene expression profile revealed a "co-upregulation" of MCP-1, 
      MCP-2, macrophage inflammatory protein-1alpha, and CD68 in OB, and whole-body 
      glucose disposal inversely correlated with the MCP-1 gene expression. After 
      hyperinsulinemia, MCP-1 and MCP-2 mRNA levels significantly increased in L, but 
      not in OB. Finally, sMCP-1 excess in the OB positively correlated with the 
      stiffer vasculature. CONCLUSIONS: These observations demonstrate similar 
      interstitial concentrations and a differential gene response to hyperinsulinemia 
      of MCP-1 in the SCAAT from L and OB individuals. In human obesity, we suggest the 
      SCAAT MCP-1 gene overexpression as a biomarker of an "inflamed" adipose organ and 
      impaired glucose metabolism.
FAU - Murdolo, Giuseppe
AU  - Murdolo G
AD  - The Lundberg Laboratory for Diabetes Research, The Sahlgrenska Academy at 
      Goteborg University, S-413 45 Goteborg, Sweden. giuseppe.murdolo@medic.gu.se
FAU - Hammarstedt, Ann
AU  - Hammarstedt A
FAU - Sandqvist, Madelene
AU  - Sandqvist M
FAU - Schmelz, Martin
AU  - Schmelz M
FAU - Herder, Christian
AU  - Herder C
FAU - Smith, Ulf
AU  - Smith U
FAU - Jansson, Per-Anders
AU  - Jansson PA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070424
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Biomarkers)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Genetic Markers)
RN  - 0 (Insulin)
SB  - IM
MH  - Adipocytes/cytology/physiology
MH  - Adult
MH  - Biomarkers
MH  - Cell Size
MH  - Chemokine CCL2/*genetics
MH  - Diabetes Mellitus, Type 2/*genetics/immunology/physiopathology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/immunology
MH  - *Genetic Markers
MH  - Humans
MH  - Hyperinsulinism/genetics/immunology/physiopathology
MH  - Insulin/*blood
MH  - Insulin Resistance
MH  - Male
MH  - Middle Aged
MH  - Obesity/genetics/immunology/physiopathology
MH  - Subcutaneous Fat/*physiology
EDAT- 2007/04/26 09:00
MHDA- 2007/08/29 09:00
CRDT- 2007/04/26 09:00
PHST- 2007/04/26 09:00 [pubmed]
PHST- 2007/08/29 09:00 [medline]
PHST- 2007/04/26 09:00 [entrez]
AID - jc.2006-2814 [pii]
AID - 10.1210/jc.2006-2814 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2007 Jul;92(7):2688-95. doi: 10.1210/jc.2006-2814. Epub 
      2007 Apr 24.