PMID- 17456591
OWN - NLM
STAT- MEDLINE
DCOM- 20070511
LR  - 20131121
IS  - 1099-8004 (Print)
IS  - 1099-8004 (Linking)
VI  - 8
IP  - 4
DP  - 2007 Apr
TI  - The dose-dependent effects of chronic iron overload on the production of oxygen 
      free radicals and vitamin E concentrations in the liver of a murine model.
PG  - 300-4
AB  - Genetic disorders of iron metabolism such as primary and secondary 
      hemochromatosis affect thousands of individuals worldwide and are major causes of 
      liver dysfunction, morbidity, and mortality. Although the exact mechanism of 
      hepatic injury associated with these genetic disorders is not fully understood, 
      the propagation of excess concentrations of iron-catalyzed oxygen free radicals 
      (OFRs) may play a role. The authors hypothesized that chronic iron burden would 
      result in dose-dependent (a) increases in hepatic iron stores, (b) increases in 
      hepatic OFR-mediated hepatic cellular injury as quantified by the cytotoxic 
      aldehydes malondialdehyde (MDA) and hexanal, and (c) decreases in protective 
      antioxidant reserve status as quantified by plasma vitamin E (alpha-tocopherol) 
      levels in a murine model. Twenty B(6)D(2)F1 male mice were randomized to the (a) 
      saline control (0.05 mL intraperiotoneal [i.p.]/mouse/day, n = 5), (b) 100 mg 
      total iron burden (n = 5), (c) 200 mg total iron burden (n = 5), or (d) 400 mg 
      total iron burden (n = 5) group. Iron burden was achieved by daily injections of 
      iron dextran (Imferon, 0.05 mL i.p./mouse/day). In comparison to control mice and 
      in support of the hypothesis, the authors observed significant dose-dependent 
      increases in total hepatic iron burden (p < .001) with corresponding increases in 
      MDA and hexanal concentrations (p < .001) and decreases in the protective plasma 
      antioxidant vitamin E (p < .001). These findings suggest that iron-catalyzed 
      OFR-mediated damage may play a role in damaging the liver in chronic states of 
      iron burden.
FAU - McCullough, Karey D
AU  - McCullough KD
AD  - Nursing Faculty, Nipissing University, North Bay, Ontario, Canada.
FAU - Bartfay, Wally J
AU  - Bartfay WJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biol Res Nurs
JT  - Biological research for nursing
JID - 9815758
RN  - 0 (Aldehydes)
RN  - 0 (Free Radicals)
RN  - 1406-18-4 (Vitamin E)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 9004-66-4 (Iron-Dextran Complex)
RN  - 9DC2K31JJQ (n-hexanal)
SB  - IM
MH  - Aldehydes/analysis/metabolism
MH  - Animals
MH  - Chronic Disease
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Drug Residues/analysis/metabolism
MH  - Free Radicals/analysis/metabolism
MH  - Gas Chromatography-Mass Spectrometry
MH  - Injections, Intraperitoneal
MH  - *Iron Overload/chemically induced/metabolism/pathology
MH  - Iron-Dextran Complex/*adverse effects
MH  - *Liver/chemistry/metabolism
MH  - Male
MH  - Malondialdehyde/analysis/metabolism
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Random Allocation
MH  - Spectrophotometry, Atomic
MH  - *Vitamin E/analysis/metabolism
EDAT- 2007/04/26 09:00
MHDA- 2007/05/12 09:00
CRDT- 2007/04/26 09:00
PHST- 2007/04/26 09:00 [pubmed]
PHST- 2007/05/12 09:00 [medline]
PHST- 2007/04/26 09:00 [entrez]
AID - 8/4/300 [pii]
AID - 10.1177/109980040629873 [doi]
PST - ppublish
SO  - Biol Res Nurs. 2007 Apr;8(4):300-4. doi: 10.1177/109980040629873.