PMID- 17456785
OWN - NLM
STAT- MEDLINE
DCOM- 20070905
LR  - 20191210
IS  - 0026-895X (Print)
IS  - 0026-895X (Linking)
VI  - 72
IP  - 2
DP  - 2007 Aug
TI  - Transcriptional regulation of brain-derived neurotrophic factor in the amygdala 
      during consolidation of fear memory.
PG  - 350-8
AB  - We have demonstrated previously that brain-derived neurotrophic factor (BDNF) 
      signaling in the amygdala is required for the consolidation of fear memory. This 
      study is designed to characterize the signal cascades by which fear conditioning 
      modulates transcriptional and translational expression of BDNF. Real-time reverse 
      transcription-coupled polymerase chain reaction showed a significant increase in 
      BDNF exon I- and III-containing mRNA in the amygdala of fear-conditioned rats, 
      indicating that fear conditioning was capable of up-regulating BDNF mRNA. 
      Bilateral administration of actinomycin D or anisomycin to the amygdala 
      attenuated conditioning-induced increase in BDNF protein. Inhibitors for 
      N-methyl-d-aspartate (NMDA) receptor, L-type voltage-dependent calcium channel 
      (L-VDCC), adenylyl cyclase, cAMP-dependent protein kinase (PKA), and 
      calcium/calmodulin-dependent kinase IV (CaMKIV) significantly reduced the 
      increase. Moreover, DNA affinity precipitation and chromatin immunoprecipitation 
      assays showed that phosphorylated cAMP response element-binding protein (p-CREB) 
      binding activity in the proximal region of BDNF promoter I and III was 
      significantly increased after fear conditioning. Intra-amygdala administration of 
      cAMP response element decoy DNA before training impaired fear learning. Taken 
      together, these results suggest that calcium influx through NMDA receptors and 
      L-VDCCs during fear conditioning activates PKA and CaMKIV resulting in CREB 
      phosphorylation. The phosphorylated CREB binds to BDNF promoter and up-regulates 
      the expression of BDNF in the amygdala, which helps the consolidation of fear 
      memory.
FAU - Ou, Li-Chin
AU  - Ou LC
AD  - Institute of Basic Medical Sciences, National Cheng-Kung University, Tainan, 
      Taiwan.
FAU - Gean, Po-Wu
AU  - Gean PW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070424
PL  - United States
TA  - Mol Pharmacol
JT  - Molecular pharmacology
JID - 0035623
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium Channels, L-Type)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
SB  - IM
MH  - Amygdala/*metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Calcium Channels, L-Type/physiology
MH  - Conditioning, Psychological
MH  - *Fear
MH  - Male
MH  - *Memory
MH  - Promoter Regions, Genetic
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/physiology
MH  - Signal Transduction
MH  - *Transcription, Genetic
EDAT- 2007/04/26 09:00
MHDA- 2007/09/06 09:00
CRDT- 2007/04/26 09:00
PHST- 2007/04/26 09:00 [pubmed]
PHST- 2007/09/06 09:00 [medline]
PHST- 2007/04/26 09:00 [entrez]
AID - mol.107.034934 [pii]
AID - 10.1124/mol.107.034934 [doi]
PST - ppublish
SO  - Mol Pharmacol. 2007 Aug;72(2):350-8. doi: 10.1124/mol.107.034934. Epub 2007 Apr 
      24.