PMID- 17459161
OWN - NLM
STAT- MEDLINE
DCOM- 20070530
LR  - 20220331
IS  - 1465-993X (Electronic)
IS  - 1465-9921 (Print)
IS  - 1465-9921 (Linking)
VI  - 8
IP  - 1
DP  - 2007 Apr 25
TI  - Genomic analysis of human lung fibroblasts exposed to vanadium pentoxide to 
      identify candidate genes for occupational bronchitis.
PG  - 34
AB  - BACKGROUND: Exposure to vanadium pentoxide (V2O5) is a cause of occupational 
      bronchitis. We evaluated gene expression profiles in cultured human lung 
      fibroblasts exposed to V2O5 in vitro in order to identify candidate genes that 
      could play a role in inflammation, fibrosis, and repair during the pathogenesis 
      of V2O5-induced bronchitis. METHODS: Normal human lung fibroblasts were exposed 
      to V2O5 in a time course experiment. Gene expression was measured at various time 
      points over a 24 hr period using the Affymetrix Human Genome U133A 2.0 Array. 
      Selected genes that were significantly changed in the microarray experiment were 
      validated by RT-PCR. RESULTS: V2O5 altered more than 1,400 genes, of which ~300 
      were induced while >1,100 genes were suppressed. Gene ontology categories (GO) 
      categories unique to induced genes included inflammatory response and immune 
      response, while GO categories unique to suppressed genes included ubiquitin cycle 
      and cell cycle. A dozen genes were validated by RT-PCR, including growth factors 
      (HBEGF, VEGF, CTGF), chemokines (IL8, CXCL9, CXCL10), oxidative stress response 
      genes (SOD2, PIPOX, OXR1), and DNA-binding proteins (GAS1, STAT1). CONCLUSION: 
      Our study identified a variety of genes that could play pivotal roles in 
      inflammation, fibrosis and repair during V2O5-induced bronchitis. The induction 
      of genes that mediate inflammation and immune responses, as well as suppression 
      of genes involved in growth arrest appear to be important to the lung fibrotic 
      reaction to V2O5.
FAU - Ingram, Jennifer L
AU  - Ingram JL
AD  - The Hamner Institutes for Health Sciences, Research Triangle Park, North Carolina 
      27709, USA. jennifer.ingram@duke.edu <jennifer.ingram@duke.edu>
FAU - Antao-Menezes, Aurita
AU  - Antao-Menezes A
FAU - Turpin, Elizabeth A
AU  - Turpin EA
FAU - Wallace, Duncan G
AU  - Wallace DG
FAU - Mangum, James B
AU  - Mangum JB
FAU - Pluta, Linda J
AU  - Pluta LJ
FAU - Thomas, Russell S
AU  - Thomas RS
FAU - Bonner, James C
AU  - Bonner JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070425
PL  - England
TA  - Respir Res
JT  - Respiratory research
JID - 101090633
RN  - 0 (Proteome)
RN  - 0 (Vanadium Compounds)
RN  - BVG363OH7A (vanadium pentoxide)
SB  - IM
MH  - Bronchitis/*chemically induced/genetics/*metabolism
MH  - Cells, Cultured
MH  - Chromosome Mapping/methods
MH  - Fibroblasts/*drug effects/*metabolism
MH  - Humans
MH  - Occupational Diseases/genetics/*metabolism
MH  - Occupational Exposure/adverse effects
MH  - Oligonucleotide Array Sequence Analysis/methods
MH  - Proteome/genetics/*metabolism
MH  - Vanadium Compounds/*poisoning
PMC - PMC1865536
EDAT- 2007/04/27 09:00
MHDA- 2007/05/31 09:00
PMCR- 2007/04/25
CRDT- 2007/04/27 09:00
PHST- 2006/12/05 00:00 [received]
PHST- 2007/04/25 00:00 [accepted]
PHST- 2007/04/27 09:00 [pubmed]
PHST- 2007/05/31 09:00 [medline]
PHST- 2007/04/27 09:00 [entrez]
PHST- 2007/04/25 00:00 [pmc-release]
AID - 1465-9921-8-34 [pii]
AID - 10.1186/1465-9921-8-34 [doi]
PST - epublish
SO  - Respir Res. 2007 Apr 25;8(1):34. doi: 10.1186/1465-9921-8-34.