PMID- 17459645
OWN - NLM
STAT- MEDLINE
DCOM- 20071003
LR  - 20131121
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 44
IP  - 3
DP  - 2007 Jul 27
TI  - High-performance liquid chromatographic determination and pharmacokinetic study 
      of vitexin-2''-O-rhamnoside in rat plasma after intravenous administration.
PG  - 802-6
AB  - A simple and specific high-performance liquid chromatographic (HPLC) method was 
      developed for the pharmacokinetic study of vitexin-2''-O-rhamnoside (VOR) in rat 
      after intravenous administration. The plasma samples were deproteinized with 
      methanol after addition of internal standard (i.s.) hesperidin. HPLC analysis was 
      performed on a Diamonsil ODS C18 analytical column, using acetonitrile-0.3% 
      formic acid (20:80, v/v) as the mobile phase with UV detection at 270 nm. The 
      standard curve was linear over the range of 0.1070-21.41 microg/mL in rat plasma. 
      The average extraction recovery of VOR was 97.9+/-3.1%, and the relative standard 
      deviations (R.S.D.s) of the intra- and inter-day precisions were no more than 7.4 
      and 8.5%, respectively. The lower limit of quantification (LLOQ) was 0.1070 
      microg/mL. The AUC of VOR was proportional to the dose after intravenous 
      administration of 15, 30, 60 and 120 mg/kg body weight, and the elimination 
      half-life (t1/2beta), systemic clearance (Cl) and apparent volume of distribution 
      (Vc) were not significantly different among the four doses, and all the results 
      indicated that the pharmacokinetics of VOR in rat obeyed first-order kinetics.
FAU - Ying, Xixiang
AU  - Ying X
AD  - School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 
      Shenyang, Liaoning Province 110016, PR China.
FAU - Gao, Shuo
AU  - Gao S
FAU - Zhu, Wenliang
AU  - Zhu W
FAU - Bi, Yujin
AU  - Bi Y
FAU - Qin, Feng
AU  - Qin F
FAU - Li, Xiaoqin
AU  - Li X
FAU - Li, Famei
AU  - Li F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070324
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Plant Extracts)
RN  - 32426-34-9 (vitexin rhamnoside)
RN  - 7V515PI7F6 (Apigenin)
RN  - E750O06Y6O (Hesperidin)
SB  - IM
MH  - Animals
MH  - Apigenin/administration & dosage/blood/chemistry/*pharmacokinetics
MH  - Area Under Curve
MH  - Calibration
MH  - Chromatography, High Pressure Liquid/instrumentation/*methods
MH  - Crataegus/chemistry
MH  - Dose-Response Relationship, Drug
MH  - Drug Stability
MH  - Freezing
MH  - Half-Life
MH  - Hesperidin/chemistry
MH  - Injections, Intravenous
MH  - Male
MH  - Metabolic Clearance Rate
MH  - Molecular Structure
MH  - Plant Extracts/chemistry
MH  - Plant Leaves/chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Temperature
MH  - Time Factors
EDAT- 2007/04/27 09:00
MHDA- 2007/10/04 09:00
CRDT- 2007/04/27 09:00
PHST- 2006/11/13 00:00 [received]
PHST- 2007/03/10 00:00 [revised]
PHST- 2007/03/16 00:00 [accepted]
PHST- 2007/04/27 09:00 [pubmed]
PHST- 2007/10/04 09:00 [medline]
PHST- 2007/04/27 09:00 [entrez]
AID - S0731-7085(07)00182-3 [pii]
AID - 10.1016/j.jpba.2007.03.015 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2007 Jul 27;44(3):802-6. doi: 10.1016/j.jpba.2007.03.015. 
      Epub 2007 Mar 24.