PMID- 17460296
OWN - NLM
STAT- MEDLINE
DCOM- 20070613
LR  - 20070426
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 48
IP  - 5
DP  - 2007 May
TI  - Neuroprotection in the juvenile rat model of light-induced retinopathy: evidence 
      suggesting a role for FGF-2 and CNTF.
PG  - 2311-20
AB  - PURPOSE: In a former study, it was demonstrated that the retina of juvenile 
      Sprague-Dawley (SD) rat has a remarkable intrinsic resistance to light-induced 
      retinopathy compared with the adult retina. The purpose of the present study was 
      to investigate the cellular and molecular mechanisms underlying this endogenous 
      resistance to light-induced damage. METHODS: Juvenile SD rats were exposed for 6 
      (from P14 to P20) or 14 (from P14 to P28) days to a bright, cyclic, luminous 
      environment of 10,000 lux. Retinal histology was examined immediately after 
      exposure to light or at 2 months of age, and photoreceptor cell death was 
      quantified by measuring the thickness of the outer nuclear layer (ONL) and by 
      TUNEL assays. Changes in protein levels and cellular localization of fibroblast 
      growth factor (FGF)-2, ciliary neurotrophic factor (CNTF), and brain-derived 
      neurotrophic factor (BDNF) were determined by Western blot analysis and retinal 
      immunohistochemistry, respectively. RESULTS: The data demonstrate that although 
      the rate of photoreceptor loss was different after 6 and 14 days of exposure to 
      light, similar ONL thickness was reached at 2 months of age--that is, 4 to 5 
      weeks after exposure to light. A large number of TUNEL-positive photoreceptors 
      was visualized immediately after 6 and 14 days of exposure to light, reflecting 
      the intense cell death that was occurring in the ONL. Western blot analysis 
      showed that exposure to light induced a strong upregulation of the neurotrophic 
      factors FGF-2 and CNTF in juvenile retinas, whereas no change in BDNF protein 
      expression was noted. Of interest, after exposure to light, endogenous FGF-2 and 
      CNTF were selectively upregulated in Muller cells. CONCLUSIONS: The results show 
      that endogenous expression of FGF-2 and CNTF by Muller glia may play a role in 
      protecting the juvenile retina from light-induced damage.
FAU - Joly, Sandrine
AU  - Joly S
AD  - Departments of Biological Sciences, University of Montreal, Quebec, Canada.
FAU - Pernet, Vincent
AU  - Pernet V
FAU - Chemtob, Sylvain
AU  - Chemtob S
FAU - Di Polo, Adriana
AU  - Di Polo A
FAU - Lachapelle, Pierre
AU  - Lachapelle P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Neuroprotective Agents)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Death
MH  - Ciliary Neurotrophic Factor/*metabolism
MH  - Cytoprotection
MH  - Disease Models, Animal
MH  - Female
MH  - Fibroblast Growth Factor 2/*metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - In Situ Nick-End Labeling
MH  - Microscopy, Fluorescence
MH  - Neuroprotective Agents/*metabolism
MH  - Pregnancy
MH  - Radiation Injuries, Experimental/*metabolism/pathology/prevention & control
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retina/metabolism/pathology/*radiation effects
MH  - Retinal Degeneration/*metabolism/pathology/prevention & control
MH  - Up-Regulation
EDAT- 2007/04/27 09:00
MHDA- 2007/06/15 09:00
CRDT- 2007/04/27 09:00
PHST- 2007/04/27 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2007/04/27 09:00 [entrez]
AID - 48/5/2311 [pii]
AID - 10.1167/iovs.06-1205 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2007 May;48(5):2311-20. doi: 10.1167/iovs.06-1205.