PMID- 17460897
OWN - NLM
STAT- MEDLINE
DCOM- 20100506
LR  - 20181201
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 23
IP  - 2
DP  - 2007 Mar
TI  - [Human bone marrow mesenchymal stem cells differentiated into dopaminergenic 
      neurons in vitro].
PG  - 252-6
AB  - Midbrain dopamine (DA) neurons play an essential role in modulating motor 
      control. Defects in central DA neurons affect a wide range of neurological 
      disorders including Parkinson's disease (PD). The greatest motivation in the 
      field has been the potential use of DA neurons for cell transplantation therapy 
      in Parkinsonian patients. Recent studies indicated that BMSCs could differentiate 
      into DA neurons in vitro as neural stem cells (NSC) and embryonic stem cells 
      (ESC) could. However, there are no direct evidences about functional DA neurons 
      derived from BMSCs. According to the protocols which had been applicated in 
      inducing neuronal stem cells and embryonic stem cells differentiate into DA 
      neurons in vitro, the present study provides a protocol by using 50 micromol/L 
      brain derived neurotrophy factor (BDNF), 10 micromol/L forskolin (FSK) and 10 
      micromol/L dopamine (DA) to induce BMSCs differentiate into DA neurons. After 2 
      weeks of differentiation, the cells expressed the character of neurons in 
      ultrastructure. RT-PCR discovered mRNA of NSE (neuron specific enolase), Nurr1, 
      Ptx3, Lmx1b and Tyrosine hydroxylase (TH) were positive. Immunocytochemistry 
      staining indicated the ratio of TH-positive neural cells was significantly 
      increased after induced 2 weeks (24.80 +/- 3.36) % compared to that of induction 
      of 3 days (3.77 +/- 1.77) %. And the DA release was also different between 
      differentiated and undifferentiated cells detected by high performance liquid 
      chromatography (HPLC). That is to say BDNF and FSK and DA can induce BMSCs 
      differentiate into DA neurons in vitro, and the transdifferentiated cells express 
      mature neurons characters. BMSCs might be a suitable and available source for the 
      in vitro derivation of DA neurons and cell transplantation therapy in some 
      central neural system diseases such as PD.
FAU - Chai, Li-Hui
AU  - Chai LH
AD  - Laboratory for Cell and Molecular Immunology of Henan University, Institute for 
      Immunology of Henan University, Kaifeng 475004, China.
FAU - Wu, Su-Xia
AU  - Wu SX
FAU - Yan, Wen-Hai
AU  - Yan WH
FAU - Ma, Yuan-Fang
AU  - Ma YF
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 1F7A44V6OU (Colforsin)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bone Marrow Cells/*cytology/metabolism/ultrastructure
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Transdifferentiation/drug effects/genetics/*physiology
MH  - Cells, Cultured
MH  - Chromatography, High Pressure Liquid
MH  - Colforsin/pharmacology
MH  - Dopamine/metabolism/pharmacology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/metabolism/ultrastructure
MH  - Microscopy, Electron, Transmission
MH  - Middle Aged
MH  - Neurons/*cytology/metabolism/ultrastructure
MH  - Phosphopyruvate Hydratase/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tyrosine 3-Monooxygenase/genetics/metabolism
MH  - Young Adult
EDAT- 2007/04/28 09:00
MHDA- 2010/05/07 06:00
CRDT- 2007/04/28 09:00
PHST- 2007/04/28 09:00 [pubmed]
PHST- 2010/05/07 06:00 [medline]
PHST- 2007/04/28 09:00 [entrez]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2007 Mar;23(2):252-6.