PMID- 17462792
OWN - NLM
STAT- MEDLINE
DCOM- 20070726
LR  - 20091119
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 50
IP  - 6
DP  - 2007 May
TI  - NT-3 modulates BDNF and proBDNF levels in naive and kindled rat hippocampus.
PG  - 866-71
AB  - Both mature and precursor forms of neurotrophins regulate nerve development, 
      survival and plasticity. Brain-derived neurotrophic factor (BDNF) synthesis and 
      secretion in turn are regulated by neuronal activity, such as epilepsy. Further, 
      neurotrophins themselves are regulated by neurotrophin levels. Neurotrophin-3 
      (NT-3) and BDNF in particular can be co-expressed and each can regulate the 
      levels of the other. This regulation is thought to be mediated through receptor 
      tyrosine kinase (Trk) activity. It is not known whether this 
      neurotrophin-neurotrophin interaction occurs in hippocampal tissue in vivo, or 
      how it is influenced by neuronal activation. In this study, we explored the 
      reciprocal influences of intraventricular infusions of NT-3 and BDNF in naive and 
      kindled hippocampi of rats using Western blotting. We confirm that hippocampal 
      kindling resulted in a significant increase in levels of BDNF both in cytochrome 
      C (control) infused and NT-3 infused kindled rats. However, NT-3 infusion 
      significantly reduced BDNF levels in both kindled and non-kindled hippocampi 
      compared to their cytochrome C infused counterparts. These results are consistent 
      with our earlier studies demonstrating lowered levels of TrkA and TrkC (NGF 
      modulates BDNF levels via TrkA) following chronic NT-3 infusion. Although 
      kindling led to an increase in BDNF, this was not accompanied by any detectable 
      change in the levels of proBDNF. However, there was a significant increase in 
      proBDNF following NT-3 infusions, suggesting NT-3 may reduce proBDNF processing. 
      In contrast, neither NT-3 nor proNT-3 levels were affected by kindling or chronic 
      BDNF infusions, consistent with down-regulation of TrkB by chronic BDNF infusion. 
      Thus, modulation of BDNF by NT-3, likely mediated by Trk receptors, occurs in 
      naive and kindled adult rat hippocampus.
FAU - Ullal, G R
AU  - Ullal GR
AD  - Department of Psychology, Neuroscience and Behaviour, McMaster University, 
      Hamilton, Ontario L8N 3Z5, Canada.
FAU - Michalski, B
AU  - Michalski B
FAU - Xu, B
AU  - Xu B
FAU - Racine, R J
AU  - Racine RJ
FAU - Fahnestock, M
AU  - Fahnestock M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070307
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Actins)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Protein Precursors)
RN  - 0 (brain-derived neurotrophic factor precursor)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkC)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*metabolism/pharmacology
MH  - Cytochromes c/metabolism
MH  - Densitometry
MH  - Down-Regulation/drug effects
MH  - Hippocampus/*metabolism
MH  - Kindling, Neurologic/*physiology
MH  - Male
MH  - Neurotrophin 3/*physiology
MH  - Protein Precursors/*metabolism
MH  - Rats
MH  - Rats, Long-Evans
MH  - Receptor, trkA/metabolism
MH  - Receptor, trkC/metabolism
EDAT- 2007/04/28 09:00
MHDA- 2007/07/27 09:00
CRDT- 2007/04/28 09:00
PHST- 2006/11/03 00:00 [received]
PHST- 2007/02/09 00:00 [revised]
PHST- 2007/02/26 00:00 [accepted]
PHST- 2007/04/28 09:00 [pubmed]
PHST- 2007/07/27 09:00 [medline]
PHST- 2007/04/28 09:00 [entrez]
AID - S0197-0186(07)00059-9 [pii]
AID - 10.1016/j.neuint.2007.02.009 [doi]
PST - ppublish
SO  - Neurochem Int. 2007 May;50(6):866-71. doi: 10.1016/j.neuint.2007.02.009. Epub 
      2007 Mar 7.