PMID- 17463054
OWN - NLM
STAT- MEDLINE
DCOM- 20070911
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 148
IP  - 8
DP  - 2007 Aug
TI  - Androgen-dependent regulation of brain-derived neurotrophic factor and tyrosine 
      kinase B in the sexually dimorphic spinal nucleus of the bulbocavernosus.
PG  - 3655-65
AB  - Castration of adult male rats causes the dendrites of androgen-sensitive 
      motoneurons of the spinal nucleus of the bulbocavernosus (SNB) to retract. 
      Brain-derived neurotrophic factor (BDNF), via activation of tyrosine receptor 
      kinase B (trkB), has been implicated in mediating androgen effects on SNB 
      dendrites. We used in situ hybridization to demonstrate that SNB motoneurons in 
      gonadally intact adult male rats contain mRNA for both BDNF and trkB. Two weeks 
      after gonadectomy, both transcripts were significantly decreased in SNB 
      motoneurons but not in the non-androgen-responsive motoneurons of the adjacent 
      retrodorsolateral nucleus (RDLN). In a second experiment, target perineal and 
      foot muscles of SNB and RDLN motoneurons, respectively, were injected with the 
      retrograde tracer Fluorogold, and then immunocytochemistry was performed to 
      examine the distribution of BDNF and trkB proteins in SNB and RDLN motoneurons 
      and their glutamatergic afferents. Confocal analysis revealed that gonadectomy 
      induces a loss of BDNF protein in SNB dendrites but not in RDLN dendrites. 
      Testosterone treatment of castrates prevented the loss of BDNF from SNB 
      dendrites. Confocal analysis also revealed trkB protein in SNB and RDLN dendrites 
      and in their glutamatergic afferents. Gonadectomy had no discernable effect on 
      trkB protein in SNB or RDLN motoneurons or in their glutamatergic afferents. 
      These results suggest that androgen maintains a BDNF-signaling pathway in SNB 
      motoneurons that may underlie the maintenance of dendritic structure and synaptic 
      signaling.
FAU - Ottem, Erich N
AU  - Ottem EN
AD  - Neuroscience Program, 108 Giltner Hall, Michigan State University, East Lansing, 
      Michigan 48824, USA. ottem@msu.edu
FAU - Beck, Laurel A
AU  - Beck LA
FAU - Jordan, Cynthia L
AU  - Jordan CL
FAU - Breedlove, S Marc
AU  - Breedlove SM
LA  - eng
GR  - NS045195/NS/NINDS NIH HHS/United States
GR  - NS28421/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070426
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt)
RN  - 0 (Androgens)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Stilbamidines)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Androgens/*metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - Dendrites/physiology
MH  - Fluorescent Dyes
MH  - Glutamic Acid/physiology
MH  - Lumbosacral Region
MH  - Male
MH  - Motor Neurons/*physiology/ultrastructure
MH  - Neurons, Afferent/physiology/ultrastructure
MH  - Orchiectomy
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/*genetics
MH  - Sex Characteristics
MH  - Spinal Cord/cytology/*physiology
MH  - Stilbamidines
EDAT- 2007/04/28 09:00
MHDA- 2007/09/12 09:00
CRDT- 2007/04/28 09:00
PHST- 2007/04/28 09:00 [pubmed]
PHST- 2007/09/12 09:00 [medline]
PHST- 2007/04/28 09:00 [entrez]
AID - en.2007-0308 [pii]
AID - 10.1210/en.2007-0308 [doi]
PST - ppublish
SO  - Endocrinology. 2007 Aug;148(8):3655-65. doi: 10.1210/en.2007-0308. Epub 2007 Apr 
      26.