PMID- 17470397
OWN - NLM
STAT- MEDLINE
DCOM- 20081002
LR  - 20070719
IS  - 1050-4648 (Print)
IS  - 1050-4648 (Linking)
VI  - 23
IP  - 3
DP  - 2007 Sep
TI  - Molecular cloning and expression of a Toll receptor in the giant tiger shrimp, 
      Penaeus monodon.
PG  - 504-13
AB  - Invertebrates rely completely for their protection against pathogens on the 
      innate immune system. This non-self-recognition is activated by microbial cell 
      wall components with unique conserved molecular patterns. Pathogen-associated 
      molecular patterns (PAMPs) are recognised by pattern recognition receptors 
      (PRRs). Toll and its mammalian homologs Toll-like receptors are cell-surface 
      receptors acting as PRRs and involved in the signalling pathway implicated in 
      their immune response. Here we describe a novel partial Toll receptor gene cloned 
      from a gill library of the giant tiger shrimp, Penaeus monodon, using primers 
      based on the highly conserved Toll/IL-1R (TIR) domain. The deduced amino acid 
      sequence of the P. monodon Toll (PmToll) shows 59% similarity to a Toll-related 
      protein of Apis mellifera. Analysis of the LRRs of shrimp Toll contained no 
      obvious PAMP-binding insertions. Phylogenetic analysis with the insect Toll 
      family shows clustering with Toll1 and Toll5 gene products, and it is less 
      related to Toll3 and Toll4. Furthermore, RT-qPCR shows that PmToll is 
      constitutively expressed in gut, gill and hepatopancreas. Challenge with white 
      spot syndrome virus (WSSV) shows equal levels of expression in these organs. A 
      role in the defence mechanism is discussed. In conclusion, shrimp possess at 
      least one Toll receptor that might be involved in immune defence.
FAU - Arts, Joop A J
AU  - Arts JA
AD  - Cell Biology and Immunology Group, Wageningen University, Marijkeweg 40, 6709 PG 
      Wageningen, The Netherlands. joop.arts@wur.nl
FAU - Cornelissen, Ferry H J
AU  - Cornelissen FH
FAU - Cijsouw, Tony
AU  - Cijsouw T
FAU - Hermsen, Trudi
AU  - Hermsen T
FAU - Savelkoul, Huub F J
AU  - Savelkoul HF
FAU - Stet, Rene J M
AU  - Stet RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20060919
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (DNA, Complementary)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry
MH  - *Gene Expression Regulation
MH  - Penaeidae/*genetics/*metabolism/virology
MH  - Phylogeny
MH  - Sequence Alignment
MH  - Toll-Like Receptors/chemistry/*genetics
MH  - White spot syndrome virus 1/physiology
EDAT- 2007/05/02 09:00
MHDA- 2008/10/03 09:00
CRDT- 2007/05/02 09:00
PHST- 2006/06/08 00:00 [received]
PHST- 2006/08/24 00:00 [revised]
PHST- 2006/08/25 00:00 [accepted]
PHST- 2007/05/02 09:00 [pubmed]
PHST- 2008/10/03 09:00 [medline]
PHST- 2007/05/02 09:00 [entrez]
AID - S1050-4648(06)00144-6 [pii]
AID - 10.1016/j.fsi.2006.08.018 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2007 Sep;23(3):504-13. doi: 10.1016/j.fsi.2006.08.018. 
      Epub 2006 Sep 19.