PMID- 17472628
OWN - NLM
STAT- MEDLINE
DCOM- 20070524
LR  - 20220409
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 9
IP  - 5
DP  - 2007 May
TI  - Microbial community structure of ethanol type fermentation in bio-hydrogen 
      production.
PG  - 1112-25
AB  - Three continuous stirred-tank reactors (CSTRs) were used for H(2) production from 
      molasses wastewater at influent pH of 6.0-6.5 (reactor A), 5.5-6.0 (reactor B), 
      or 4.0-4.5 (reactor C). After operation for 28 days, the microbial community 
      formed ethanol type (C), propionate type (A) and ethanol-butyrate-mixed type (B) 
      fermentation. The H(2) production rate was the highest for ethanol type 
      fermentation, 0.40 l (g VSS)(-1) day(-1) or 0.45 l H(2) (g COD removed)(-1). 
      Microbial community dynamics and diversity were analysed using double-gradient 
      denaturing gradient gel electrophoresis (DG-DGGE). Denaturing gradient gel 
      electrophoresis profiles indicated that the community structures changed quickly 
      in the first 14 days. Phylogenetic analysis indicated that the dominant bacterial 
      groups were low G+C Gram-positive bacteria, Bacteroides, gamma-Proteobacteria and 
      Actinobacteria; alpha-Proteobacteria, beta-Proteobacteria, delta-Proteobacteria 
      and Spirochaetes were also presented as minor groups in the three reactors. 
      H(2)-producing bacteria were affiliated with Ethanoligenens, Acetanaerobacterium, 
      Clostridium, Megasphaera, Citrobacter and Bacteroides. An ethanol-based 
      H(2)-producing bacterium, Ethanoligenens harbinense CGMCC1152, was isolated from 
      reactor C and visualized using fluorescence in situ hybridization (FISH) to be 
      19% of the eubacteria in reactor C. In addition, isoenzyme activity staining for 
      alcohol dehydrogenase (ADH) supported that the majority of ethanol-producing 
      bacteria were affiliated with Ethanoligenens in the microbial community.
FAU - Ren, Nanqi
AU  - Ren N
AD  - School of Municipal and Environmental Engineering, Harbin Institute of 
      Technology, Harbin 150090, China.
FAU - Xing, Defeng
AU  - Xing D
FAU - Rittmann, Bruce E
AU  - Rittmann BE
FAU - Zhao, Lihua
AU  - Zhao L
FAU - Xie, Tianhui
AU  - Xie T
FAU - Zhao, Xin
AU  - Zhao X
LA  - eng
SI  - GENBANK/AY833426
SI  - GENBANK/AY883097
SI  - GENBANK/AY883098
SI  - GENBANK/AY883099
SI  - GENBANK/AY883100
SI  - GENBANK/AY883101
SI  - GENBANK/AY883102
SI  - GENBANK/AY883103
SI  - GENBANK/AY883104
SI  - GENBANK/AY883105
SI  - GENBANK/AY883106
SI  - GENBANK/AY883107
SI  - GENBANK/AY883108
SI  - GENBANK/AY883109
SI  - GENBANK/AY883110
SI  - GENBANK/AY883111
SI  - GENBANK/AY883112
SI  - GENBANK/AY883113
SI  - GENBANK/AY883114
SI  - GENBANK/AY883115
SI  - GENBANK/AY883116
SI  - GENBANK/AY883117
SI  - GENBANK/AY883118
SI  - GENBANK/AY883119
SI  - GENBANK/AY883120
SI  - GENBANK/AY883121
SI  - GENBANK/AY883122
SI  - GENBANK/AY883123
SI  - GENBANK/AY883124
SI  - GENBANK/AY883125
SI  - GENBANK/AY883126
SI  - GENBANK/AY883127
SI  - GENBANK/AY883128
SI  - GENBANK/AY883129
SI  - GENBANK/AY883130
SI  - GENBANK/AY883131
SI  - GENBANK/AY883132
SI  - GENBANK/AY883133
SI  - GENBANK/AY883134
SI  - GENBANK/AY883135
SI  - GENBANK/AY883136
SI  - GENBANK/AY883137
SI  - GENBANK/AY883138
SI  - GENBANK/AY883139
SI  - GENBANK/AY883140
SI  - GENBANK/AY883141
SI  - GENBANK/AY883142
SI  - GENBANK/AY883143
SI  - GENBANK/AY883144
SI  - GENBANK/AY883145
SI  - GENBANK/AY883146
SI  - GENBANK/AY883147
SI  - GENBANK/AY883148
SI  - GENBANK/AY883149
SI  - GENBANK/AY883150
SI  - GENBANK/AY883151
SI  - GENBANK/AY883152
SI  - GENBANK/AY883153
SI  - GENBANK/AY883154
SI  - GENBANK/AY883155
SI  - GENBANK/AY883156
SI  - GENBANK/AY883157
SI  - GENBANK/AY883158
SI  - GENBANK/AY883159
SI  - GENBANK/AY883160
SI  - GENBANK/AY883161
SI  - GENBANK/AY883162
SI  - GENBANK/AY883163
SI  - GENBANK/AY883164
SI  - GENBANK/AY883165
SI  - GENBANK/AY883166
SI  - GENBANK/AY953329
SI  - GENBANK/AY953330
SI  - GENBANK/AY953331
SI  - GENBANK/AY953332
SI  - GENBANK/AY953333
SI  - GENBANK/AY953334
SI  - GENBANK/AY953335
SI  - GENBANK/AY953336
SI  - GENBANK/AY953337
SI  - GENBANK/AY953338
SI  - GENBANK/AY953339
SI  - GENBANK/AY953340
SI  - GENBANK/AY953341
SI  - GENBANK/AY953342
SI  - GENBANK/AY953343
SI  - GENBANK/AY953344
SI  - GENBANK/AY953345
SI  - GENBANK/AY953346
SI  - GENBANK/AY953347
SI  - GENBANK/AY953348
SI  - GENBANK/AY953349
SI  - GENBANK/AY953350
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Water Pollutants, Chemical)
RN  - 3K9958V90M (Ethanol)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Bacteria, Anaerobic/classification/*metabolism
MH  - Bioreactors
MH  - Conservation of Energy Resources/*methods
MH  - DNA Fingerprinting
MH  - *Ecosystem
MH  - Ethanol/*metabolism
MH  - Fermentation
MH  - Food-Processing Industry
MH  - Hydrogen/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - In Situ Hybridization, Fluorescence
MH  - Molasses/*microbiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/classification
MH  - Waste Disposal, Fluid/methods
MH  - Water Pollutants, Chemical/*metabolism
EDAT- 2007/05/03 09:00
MHDA- 2007/05/26 09:00
CRDT- 2007/05/03 09:00
PHST- 2007/05/03 09:00 [pubmed]
PHST- 2007/05/26 09:00 [medline]
PHST- 2007/05/03 09:00 [entrez]
AID - EMI1234 [pii]
AID - 10.1111/j.1462-2920.2006.01234.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2007 May;9(5):1112-25. doi: 10.1111/j.1462-2920.2006.01234.x.