PMID- 17474899
OWN - NLM
STAT- MEDLINE
DCOM- 20070809
LR  - 20070503
IS  - 1751-5521 (Print)
IS  - 1751-5521 (Linking)
VI  - 29
IP  - 3
DP  - 2007 Jun
TI  - FISH detection of chimerism in pediatric hematopoietic stem cell transplantation.
PG  - 208-14
AB  - Allogeneic hematopoietic stem cell transplantation (HSCT) is a well-established 
      curative therapy for various malignant and non-malignant diseases. Successful 
      outcome after allogeneic HSCT has been associated with donor chimerism (DC). 
      However, the detection of residual host cells or mixed hemopoietic chimerism (MC) 
      has indicated that donor chimerism is not obligatory following HSCT. More 
      recently, fluorescence in situ hybridization (FISH) analysis has been applied to 
      engraftment studies for the identification of polymorphic or sex chromosomes. In 
      this study, chimerism status was evaluated in 48 sex-mismatched HSCT pediatric 
      patients (17 women/31 men, mean age: 9.02 +/- 3.95 years, range: 2-19) by FISH 
      and the effect of DC or MC on outcome and long-term disease-free survival was 
      documented. The stem cell source was bone marrow in all cases. All of the donors 
      were human leucocyte antigen-identical siblings. FISH was performed on 156 
      specimens between days +13 and +1878. Donor chimerism was found in 47.9% (23/48) 
      and MC was found in 52.1% (25/48) of the patients. Fifteen of 48 (31.25%) 
      patients died, of whom 12 (80%) were MC and three patients (20%) were DC. The 
      difference in chimerism status (MC or DC) was statistically significant between 
      those patients who died and those still alive (chi(2) = 6.813; P = 0.009).
FAU - Aydin, F
AU  - Aydin F
AD  - Department of Medical Biology, Istanbul Medical Faculty, Istanbul University, 
      Istanbul, Turkey. filizkaydin@yahoo.com
FAU - Oguz, F
AU  - Oguz F
FAU - Onal, A E
AU  - Onal AE
FAU - Bilgen, H
AU  - Bilgen H
FAU - Oguz, R
AU  - Oguz R
FAU - Anak, S
AU  - Anak S
FAU - Carin, M
AU  - Carin M
FAU - Gedikoglu, G
AU  - Gedikoglu G
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Graft vs Host Disease/etiology
MH  - *Hematopoietic Stem Cell Transplantation/mortality
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Survival Analysis
MH  - *Transplantation Chimera
MH  - Transplantation, Homologous/adverse effects
EDAT- 2007/05/04 09:00
MHDA- 2007/08/10 09:00
CRDT- 2007/05/04 09:00
PHST- 2007/05/04 09:00 [pubmed]
PHST- 2007/08/10 09:00 [medline]
PHST- 2007/05/04 09:00 [entrez]
AID - CLH865 [pii]
AID - 10.1111/j.1751-553X.2007.00865.x [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2007 Jun;29(3):208-14. doi: 10.1111/j.1751-553X.2007.00865.x.