PMID- 17475242
OWN - NLM
STAT- MEDLINE
DCOM- 20070919
LR  - 20151119
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 566
IP  - 1-3
DP  - 2007 Jul 2
TI  - Intracellular calcium stores in beta-escin skinned rat and guinea-pig bladders.
PG  - 172-80
AB  - Intracellular Ca2+ stores in rat and guinea-pig bladders and taenia caecum were 
      studied in beta-escin skinned smooth muscle strips. 30 min of skinning with 40 
      microM and 80 microM beta-escin were the best parameters found to obtain good 
      calcium response curves (10(-7)-10(-4) M) in rat and guinea pig, respectively. 
      Calmodulin (1 microM) increased the calcium contractions significantly. pCa 6 was 
      used to load intracellular stores and application of carbachol (50 microM) in all 
      tissues then only contracted the tissues in the presence of 
      guanosine-5'-triphosphate (GTP; 100 microM). Inositol triphosphate (IP3; 50 
      microM), applied after pCa 6, contracted all tissues. Carbachol added after IP3 
      or heparin (1 mg/ml) no longer caused a contraction in any of them. In bladders, 
      caffeine (30 mM) but not ryanodine (5 microM) prevented the subsequent carbachol 
      contraction. A slowly rising contraction with carbachol was elicited after 
      caffeine (30 mM) or ryanodine (5 microM) in the taenia and after ryanodine in the 
      bladders. Caffeine (30 mM) suppressed the calcium response curves in all tissues. 
      Procaine (30 mM) blocked the carbachol (50 microM) contractions in bladders but 
      not in taenia. These results suggest that calcium induced calcium release (CICR) 
      and IP3 induced calcium release (IICR) release calcium from a common store in 
      bladder but two different compartments in taenia.
FAU - Tugba Durlu-Kandilci, N
AU  - Tugba Durlu-Kandilci N
AD  - Hacettepe University, Faculty of Pharmacy, Department of Pharmacology, 06100, 
      Sihhiye, Ankara, Turkey.
FAU - Brading, Alison F
AU  - Brading AF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070330
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Calmodulin)
RN  - 0 (Inositol Phosphates)
RN  - 15662-33-6 (Ryanodine)
RN  - 3G6A5W338E (Caffeine)
RN  - 4Z8Y51M438 (Procaine)
RN  - 6805-41-0 (Escin)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8Y164V895Y (Carbachol)
RN  - 9005-49-6 (Heparin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Caffeine/pharmacology
MH  - Calcium/*physiology
MH  - Calmodulin/pharmacology
MH  - Carbachol
MH  - Cecum/drug effects/*physiology
MH  - Escin/pharmacology
MH  - Guanosine Triphosphate/pharmacology
MH  - Guinea Pigs
MH  - Heparin/pharmacology
MH  - Inositol Phosphates/pharmacology
MH  - Male
MH  - Muscle Contraction/drug effects
MH  - Procaine/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Ryanodine/pharmacology
MH  - Urinary Bladder/drug effects/*physiology
EDAT- 2007/05/04 09:00
MHDA- 2007/09/20 09:00
CRDT- 2007/05/04 09:00
PHST- 2006/09/28 00:00 [received]
PHST- 2007/03/12 00:00 [revised]
PHST- 2007/03/14 00:00 [accepted]
PHST- 2007/05/04 09:00 [pubmed]
PHST- 2007/09/20 09:00 [medline]
PHST- 2007/05/04 09:00 [entrez]
AID - S0014-2999(07)00390-1 [pii]
AID - 10.1016/j.ejphar.2007.03.041 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2007 Jul 2;566(1-3):172-80. doi: 10.1016/j.ejphar.2007.03.041. 
      Epub 2007 Mar 30.