PMID- 17475245
OWN - NLM
STAT- MEDLINE
DCOM- 20070817
LR  - 20091119
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 313
IP  - 11
DP  - 2007 Jul 1
TI  - Identification and characterization of cells with high angiogenic potential and 
      transitional phenotype in calcific aortic valve.
PG  - 2326-35
AB  - Recent data suggest that angiogenesis plays an important role in the pathogenesis 
      of valvular disease. However, the cellular mechanisms underlying this process 
      remain unknown. This study aimed at identifying and characterizing the cellular 
      components responsible for pathological neovascularization in calcific aortic 
      valves (CAV). Immunohistochemical analysis of uncultured CAV tissues revealed 
      that smooth muscle alpha-actin (alpha-SMA)-positive cells, which coexpressed 
      Tie-2 and vascular endothelial growth factor receptor-2 (VEGFR-2), can be 
      identified prior to the initiation of capillary-like tube formation. In a second 
      step, leaflets of CAV and non-calcific aortic valves (NCAV) were cultured and the 
      cells involved in capillary-like tube formation were isolated. The majority of 
      these cells displayed the same phenotype as non-cultured cells identified in CAV 
      tissues, i.e., expression of alpha-SMA, Tie-2, and VEGFR-2. In comparison to 
      cells isolated from cultures of NCAV leaflets, these cells showed enhanced 
      angiogenic activity as demonstrated by migration and tube assays. The 
      coexpression of VEGFR-2 and Tie-2 together with alpha-SMA suggests both 
      endothelial and mesenchymal properties of the angiogenically activated cells 
      involved in valvular neovascularization. Hence, our findings might provide new 
      insights into the process of pathological angiogenesis in cardiac valves.
FAU - Chalajour, Fariba
AU  - Chalajour F
AD  - Department of Cardiovascular Surgery, University Heart Center-Hamburg, Martinistr 
      52, Hamburg, Germany. chalajou@uke.uni-hamburg.de
FAU - Treede, Hendrik
AU  - Treede H
FAU - Gehling, Ursula M
AU  - Gehling UM
FAU - Ebrahimnejad, Alireza
AU  - Ebrahimnejad A
FAU - Boehm, Dieter H
AU  - Boehm DH
FAU - Riemer, Robert K
AU  - Riemer RK
FAU - Ergun, Suleyman
AU  - Ergun S
FAU - Reichenspurner, Hermann
AU  - Reichenspurner H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070314
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Actins)
RN  - 0 (Antigens, CD)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 2.7.10.1 (Receptor, TIE-2)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
SB  - IM
MH  - Actins/metabolism
MH  - Antigens, CD/analysis
MH  - Aortic Valve/chemistry/metabolism/*pathology
MH  - Aortic Valve Stenosis/genetics/metabolism/*pathology
MH  - Biological Assay
MH  - Cells, Cultured
MH  - Chemotaxis
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Male
MH  - Neovascularization, Pathologic/genetics/metabolism/*pathology
MH  - Organ Culture Techniques
MH  - Phenotype
MH  - Receptor, TIE-2/metabolism
MH  - Transcription, Genetic
MH  - Vascular Endothelial Growth Factor A/pharmacology
MH  - Vascular Endothelial Growth Factor Receptor-2/metabolism
EDAT- 2007/05/04 09:00
MHDA- 2007/08/19 09:00
CRDT- 2007/05/04 09:00
PHST- 2006/08/24 00:00 [received]
PHST- 2007/01/03 00:00 [revised]
PHST- 2007/02/28 00:00 [accepted]
PHST- 2007/05/04 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2007/05/04 09:00 [entrez]
AID - S0014-4827(07)00094-8 [pii]
AID - 10.1016/j.yexcr.2007.02.033 [doi]
PST - ppublish
SO  - Exp Cell Res. 2007 Jul 1;313(11):2326-35. doi: 10.1016/j.yexcr.2007.02.033. Epub 
      2007 Mar 14.