PMID- 17477681
OWN - NLM
STAT- MEDLINE
DCOM- 20070706
LR  - 20151119
IS  - 0034-6748 (Print)
IS  - 0034-6748 (Linking)
VI  - 78
IP  - 4
DP  - 2007 Apr
TI  - Easily fabricated magnetic traps for single-cell applications.
PG  - 044301
AB  - We describe a simple and inexpensive method of fabricating single cell magnetic 
      traps within a polydimethylsiloxane (PDMS) device. These traps were developed as 
      part of an automated system that captures individual yeast cells in a 
      microfluidic device and analyzes each cell as it buds. To make the traps, PdCl(2) 
      catalyst is rubbed with vinyl foam onto plasma-patterned PDMS, and then Co-Ni-B 
      alloy is electrolessly deposited onto the catalyst at a moderate temperature. We 
      demonstrate individual yeast cell capture and estimate the capture force (1.9-4.4 
      pN) by measuring the flow speed required to remove the cell from its trap in a 
      microfluidic channel.
FAU - Koschwanez, John H
AU  - Koschwanez JH
AD  - Department of Electrical Engineering, University of Washington, Seattle, WA 
      98195-2500, USA.
FAU - Carlson, Robert H
AU  - Carlson RH
FAU - Meldrum, Deirdre R
AU  - Meldrum DR
LA  - eng
GR  - 1 R01 HG01497/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Rev Sci Instrum
JT  - The Review of scientific instruments
JID - 0405571
RN  - 0 (Alloys)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Silicones)
RN  - 5TWQ1V240M (Palladium)
RN  - 63148-62-9 (baysilon)
RN  - N9214IR8N7 (palladium chloride)
SB  - IM
MH  - Alloys/chemistry
MH  - Catalysis
MH  - Cells, Immobilized/ultrastructure
MH  - Dimethylpolysiloxanes/chemistry
MH  - Magnetics
MH  - *Microfluidic Analytical Techniques
MH  - *Microscopy, Atomic Force
MH  - Palladium/chemistry
MH  - Saccharomycetales/growth & development/*ultrastructure
MH  - Silicones/chemistry
EDAT- 2007/05/05 09:00
MHDA- 2007/07/07 09:00
CRDT- 2007/05/05 09:00
PHST- 2007/05/05 09:00 [pubmed]
PHST- 2007/07/07 09:00 [medline]
PHST- 2007/05/05 09:00 [entrez]
AID - 10.1063/1.2722400 [doi]
PST - ppublish
SO  - Rev Sci Instrum. 2007 Apr;78(4):044301. doi: 10.1063/1.2722400.