PMID- 17478127
OWN - NLM
STAT- MEDLINE
DCOM- 20071009
LR  - 20131121
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 854
IP  - 1-2
DP  - 2007 Jul 1
TI  - Determination of nitric oxide in hydrophytes using poly(methacrylic acid-ethylene 
      glycol dimethacrylate) monolith microextraction coupled to high-performance 
      liquid chromatography with fluorescence detection.
PG  - 135-42
AB  - Nitric oxide (NO) is a bioactive molecule that has recently emerged as a cellular 
      messenger in numerous physiological processes in plants. A novel high-performance 
      liquid chromatography (HPLC) method combined with poly(methacrylic acid-ethylene 
      glycol dimethacrylate) (MAA-EGDMA) monolith microextraction (PMME) is developed 
      for sensitive determination of NO in hydrophytes. NO is derivatized using a 
      fluorescent probe, 
      1,3,5,7-tetramethyl-8-(3',4'-diaminophenyl)-difluoroboradiaza-s-indacene (DAMBO), 
      and then the derivatives are extracted with PMME and analyzed by high-performance 
      liquid chromatography (HPLC) with fluorescence detection. The conditions for the 
      derivatization and the subsequent extraction of NO derivatives are optimized in 
      detail. The detection limit (S/N=3) of NO is determined to be 2x10(-12)mol L(-1). 
      Close correlation coefficient and excellent method reproducibility are obtained 
      for the analyte over a linear range of 9x10(-11)-4.5x10(-8)mol L(-1). The inter- 
      and intraday relative standard deviations (R.S.D.s) are less than 5%. The 
      proposed method is successfully applied to the determination of NO levels in 
      hydrophytes samples.
FAU - Huang, Ke-Jing
AU  - Huang KJ
AD  - Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Zhang, Min
AU  - Zhang M
FAU - Xie, Wan-Zhen
AU  - Xie WZ
FAU - Zhang, Hua-Shan
AU  - Zhang HS
FAU - Feng, Yu-Qi
AU  - Feng YQ
FAU - Wang, Hong
AU  - Wang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070419
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Ethylene Glycols)
RN  - 0 (Methacrylates)
RN  - 0 (poly(methacrylic acid-ethylene glycol dimethacrylate))
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Ethylene Glycols/*chemistry
MH  - Methacrylates/*chemistry
MH  - Nitric Oxide/*analysis
MH  - Reproducibility of Results
MH  - Spectrometry, Fluorescence/*methods
EDAT- 2007/05/05 09:00
MHDA- 2007/10/10 09:00
CRDT- 2007/05/05 09:00
PHST- 2007/01/10 00:00 [received]
PHST- 2007/04/03 00:00 [revised]
PHST- 2007/04/10 00:00 [accepted]
PHST- 2007/05/05 09:00 [pubmed]
PHST- 2007/10/10 09:00 [medline]
PHST- 2007/05/05 09:00 [entrez]
AID - S1570-0232(07)00279-6 [pii]
AID - 10.1016/j.jchromb.2007.04.012 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jul 1;854(1-2):135-42. doi: 
      10.1016/j.jchromb.2007.04.012. Epub 2007 Apr 19.