PMID- 17481971
OWN - NLM
STAT- MEDLINE
DCOM- 20071009
LR  - 20191210
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 854
IP  - 1-2
DP  - 2007 Jul 1
TI  - Validation of a rapid micellar electrokinetic capillary chromatographic method 
      for the simultaneous determination of isoniazid and pyridoxine hydrochloride in 
      pharmaceutical formulation.
PG  - 35-42
AB  - An efficient and reliable micellar electrokinetic capillary chromatography (MEKC) 
      method has been developed for the simultaneous determination of isoniazid (ISO) 
      and pyridoxine hydrochloride (PYR) in pharmaceutical formulations. A chemometric 
      two level full factorial design approach was used to search for the optimum 
      conditions of separation. Three parameters were selected for this study: the 
      buffer pH, the buffer concentration and sodium dodecyl sulphate (SDS) 
      concentrations. Resolution, peak symmetry and analysis time were established as 
      response. The two analytes were separated within 6 min with the optimized 
      conditions: 50 mM borate buffer, 25 mM SDS pH 7.8, 35 degrees C, at 50 mbar 4s 
      injection and 30 kV by using a fused silica capillary (72 cm effective length, 50 
      microm i.d.). The detection wavelength was set to 205 nm. Meloxicam was used as 
      internal standard. The method was validated with respect to stability, linearity 
      range, limit of quantitation and detection, precision, accuracy, specificity and 
      robustness. The detection limits of the method were 1.0 microg mL(-1) for ISO and 
      0.40 microg mL(-1) for PYR and the method was linear at least in the range of 
      3.0-100 microg mL(-1) for ISO and 1.0-100 microg mL(-1) for PYR with excellent 
      correlation coefficients (0.9995 for ISO and 0.9998 for PYR). Relative standard 
      deviations (R.S.D.s) of the described method ranged between 0.54 and 2.27% for 
      intra-day precision and between 0.65 and 2.69% for inter-day precision. The 
      developed method was applied to the tablet form of ISO and PYR-containing the 
      pharmaceutical preparations and the data were compared with obtained from the 
      standard addition method. No statistically significant difference was found.
FAU - Nemutlu, E
AU  - Nemutlu E
AD  - Department of Analytical Chemistry, Faculty of Pharmacy, University of Hacettepe, 
      06100 Ankara, Turkey.
FAU - Celebier, M
AU  - Celebier M
FAU - Uyar, B
AU  - Uyar B
FAU - Altinoz, S
AU  - Altinoz S
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20070412
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Pharmaceutical Preparations)
RN  - KV2JZ1BI6Z (Pyridoxine)
RN  - V83O1VOZ8L (Isoniazid)
SB  - IM
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Isoniazid/*analysis
MH  - Pharmaceutical Preparations/*chemistry
MH  - Pyridoxine/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2007/05/08 09:00
MHDA- 2007/10/10 09:00
CRDT- 2007/05/08 09:00
PHST- 2006/11/15 00:00 [received]
PHST- 2007/03/09 00:00 [revised]
PHST- 2007/03/26 00:00 [accepted]
PHST- 2007/05/08 09:00 [pubmed]
PHST- 2007/10/10 09:00 [medline]
PHST- 2007/05/08 09:00 [entrez]
AID - S1570-0232(07)00256-5 [pii]
AID - 10.1016/j.jchromb.2007.03.050 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jul 1;854(1-2):35-42. doi: 
      10.1016/j.jchromb.2007.03.050. Epub 2007 Apr 12.