PMID- 17481998
OWN - NLM
STAT- MEDLINE
DCOM- 20070719
LR  - 20090608
IS  - 0363-5023 (Print)
IS  - 0363-5023 (Linking)
VI  - 32
IP  - 5
DP  - 2007 May-Jun
TI  - Detection of apoptotic cartilage cells in symptomatic central tears of the 
      triangular fibrocartilage.
PG  - 618-22
AB  - PURPOSE: Central traumatic injuries of the triangular fibrocartilage are frequent 
      sources of ulnar wrist pain. It is unknown whether the fibrocartilage cells 
      survive this trauma in the central part of the triangular fibrocartilage. The 
      goal of this study was to determine the viability of cells in traumatic 
      triangular fibrocartilage complex (TFCC) Palmer 1A lesions to estimate the role 
      of apoptosis for the fate of fibrocartilage cells. METHODS: Twenty-two patients 
      with a symptomatic central traumatic tear in the triangular fibrocartilage were 
      included in this study. The cartilage was debrided arthroscopically, and 
      histologic sections were used for the analysis of cell viability and apoptosis. 
      Cell viability was quantified by terminal deoxyribonucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay, and apoptotic cells 
      were visualized by poly(ADP-ribose) polymerase (PARP) p85 immunohistochemistry. 
      The number of apoptotic cells was correlated with the total number of cells. 
      RESULTS: In contrast with the control patients with only minimal amounts of 
      TUNEL-positive cells, cell viability was markedly reduced in all analyzed 
      patients with TFCC Palmer 1A lesion. Likewise, the number of PARP p85-positive 
      cells was significantly increased in all patients. This indicates that the 
      observed cell death is attributed to apoptosis. Concerning the distribution of 
      apoptotic cells, the inner zone of the biospy showed much higher numbers of 
      apoptotic cells than the outer zone in both PARP and TUNEL staining. In contrast 
      to the analyzed patients, no apoptosis could be detected in the controls. 
      CONCLUSIONS: High amounts of apoptotic cartilage cells could be detected within 
      the inner part of the triangular fibrocartilage suggesting that fibrocartilage 
      cells died in response to trauma and were not quickly replaced by new viable 
      cells. PARP p85-positive cells indicate that the apoptotic cascade plays a 
      crucial role in the TFCC Palmer 1A disorder.
FAU - Unglaub, Frank
AU  - Unglaub F
AD  - Department of Hand, Plastic and Reconstructive Surgery, Burn Center, BG Trauma 
      Center Ludwigshafen, Plastic and Hand Surgery of the University of Heidelberg, 
      Ludwigshafen, Germany. funglaub@bgu-ludwigshafen.de
FAU - Fellenberg, Jorg
AU  - Fellenberg J
FAU - Germann, Guenter
AU  - Germann G
FAU - Bickert, Berthold
AU  - Bickert B
FAU - Sauerbier, Michael
AU  - Sauerbier M
FAU - Richter, Wiltrud
AU  - Richter W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Hand Surg Am
JT  - The Journal of hand surgery
JID - 7609631
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
SB  - IM
MH  - Adult
MH  - *Apoptosis
MH  - Biopsy
MH  - Cartilage/metabolism/*pathology
MH  - Case-Control Studies
MH  - Cell Survival
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Triangular Fibrocartilage/*injuries/*pathology
EDAT- 2007/05/08 09:00
MHDA- 2007/07/20 09:00
CRDT- 2007/05/08 09:00
PHST- 2006/02/06 00:00 [received]
PHST- 2007/02/24 00:00 [revised]
PHST- 2007/02/27 00:00 [accepted]
PHST- 2007/05/08 09:00 [pubmed]
PHST- 2007/07/20 09:00 [medline]
PHST- 2007/05/08 09:00 [entrez]
AID - S0363-5023(07)00306-1 [pii]
AID - 10.1016/j.jhsa.2007.02.023 [doi]
PST - ppublish
SO  - J Hand Surg Am. 2007 May-Jun;32(5):618-22. doi: 10.1016/j.jhsa.2007.02.023.