PMID- 17483188
OWN - NLM
STAT- MEDLINE
DCOM- 20071015
LR  - 20181113
IS  - 0006-3495 (Print)
IS  - 1542-0086 (Electronic)
IS  - 0006-3495 (Linking)
VI  - 93
IP  - 5
DP  - 2007 Sep 1
TI  - Two-dimensional standing wave total internal reflection fluorescence microscopy: 
      superresolution imaging of single molecular and biological specimens.
PG  - 1747-57
AB  - The development of high resolution, high speed imaging techniques allows the 
      study of dynamical processes in biological systems. Lateral resolution 
      improvement of up to a factor of 2 has been achieved using structured 
      illumination. In a total internal reflection fluorescence microscope, an 
      evanescence excitation field is formed as light is total internally reflected at 
      an interface between a high and a low index medium. The <100 nm penetration depth 
      of evanescence field ensures a thin excitation region resulting in low background 
      fluorescence. We present even higher resolution wide-field biological imaging by 
      use of standing wave total internal reflection fluorescence (SW-TIRF). Evanescent 
      standing wave (SW) illumination is used to generate a sinusoidal high spatial 
      frequency fringe pattern on specimen for lateral resolution enhancement. To 
      prevent thermal drift of the SW, novel detection and estimation of the SW phase 
      with real-time feedback control is devised for the stabilization and control of 
      the fringe phase. SW-TIRF is a wide-field superresolution technique with 
      resolution better than a fifth of emission wavelength or approximately 100 nm 
      lateral resolution. We demonstrate the performance of the SW-TIRF microscopy 
      using one- and two-directional SW illumination with a biological sample of 
      cellular actin cytoskeleton of mouse fibroblast cells as well as single 
      semiconductor nanocrystal molecules. The results confirm the superior resolution 
      of SW-TIRF in addition to the merit of a high signal/background ratio from TIRF 
      microscopy.
FAU - Chung, Euiheon
AU  - Chung E
AD  - Harvard-Massachusetts Institutes of Technology, Division of Health Sciences and 
      Technology, Department of Mechanical Engineering, Massachusetts Institute of 
      Technology, Cambridge, MA 02139, USA.
FAU - Kim, Daekeun
AU  - Kim D
FAU - Cui, Yan
AU  - Cui Y
FAU - Kim, Yang-Hyo
AU  - Kim YH
FAU - So, Peter T C
AU  - So PT
LA  - eng
GR  - P01 HL064858/HL/NHLBI NIH HHS/United States
GR  - P01 HL64858/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070504
PL  - United States
TA  - Biophys J
JT  - Biophysical journal
JID - 0370626
RN  - 0 (Actins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Polystyrenes)
SB  - IM
MH  - Actins/chemistry
MH  - Animals
MH  - Biophysics/*methods
MH  - Cytoskeleton/chemistry
MH  - Equipment Design
MH  - Fluorescent Dyes/pharmacology
MH  - Image Processing, Computer-Assisted
MH  - Mice
MH  - Microscopy, Fluorescence/*methods
MH  - Models, Theoretical
MH  - NIH 3T3 Cells
MH  - Polystyrenes/chemistry
MH  - Quantum Dots
MH  - Semiconductors
MH  - Time Factors
PMC - PMC1948056
EDAT- 2007/05/08 09:00
MHDA- 2007/10/16 09:00
PMCR- 2008/09/01
CRDT- 2007/05/08 09:00
PHST- 2007/05/08 09:00 [pubmed]
PHST- 2007/10/16 09:00 [medline]
PHST- 2007/05/08 09:00 [entrez]
PHST- 2008/09/01 00:00 [pmc-release]
AID - S0006-3495(07)71430-3 [pii]
AID - 97907 [pii]
AID - 10.1529/biophysj.106.097907 [doi]
PST - ppublish
SO  - Biophys J. 2007 Sep 1;93(5):1747-57. doi: 10.1529/biophysj.106.097907. Epub 2007 
      May 4.