PMID- 17486368
OWN - NLM
STAT- MEDLINE
DCOM- 20071227
LR  - 20181113
IS  - 0171-5216 (Print)
IS  - 0171-5216 (Linking)
VI  - 133
IP  - 9
DP  - 2007 Sep
TI  - Alcohol, smoking and human papillomavirus in laryngeal carcinoma: a Nordic 
      prospective multicenter study.
PG  - 673-8
AB  - PURPOSE: Human papillomavirus (HPV) has been linked to oropharyngeal carcinomas, 
      but its role in laryngeal squamous cell carcinoma (LSCC) is not clear. A 
      prospective multicenter study based on known tumor-cell percentage of fresh 
      frozen carcinoma biopsies was established to determine the HPV prevalence. 
      Moreover risk factors such as smoking, alcohol abuse, chronic laryngitis and 
      gastroesophageal reflux disease (GERD) were evaluated METHODS: Fresh-frozen 
      laryngeal cancer biopsies from 108 patients in Finland, Norway, and Sweden were 
      investigated. Patients whose biopsy samples contained at least 20% tumor tissue 
      (N = 69) entered the study. HPV DNA was determined with MY09/11 and GP5+/6+ 
      nested PCR and SPF10 PCR hybridization assay. Patients were examined by an ENT 
      specialist and an extensive questionnaire concerning risk factors was filled in. 
      RESULTS: Only three patients (4.4%) harbored HPV DNA in their carcinoma sample. 
      Heavy alcohol drinking was associated with an increased risk of death, 
      advanced-stage disease, and younger age at diagnosis. Chronic laryngitis, GERD, 
      and orogenital sex contacts were rare. Poor oral hygiene was not associated with 
      survival, although it correlated with heavy drinking. CONCLUSION: In our series 
      HPV was not important in LSCC. Heavy drinking led to major mortality in LSCC and 
      promoted early carcinogenesis.
FAU - Koskinen, Walter J
AU  - Koskinen WJ
AD  - Department of Virology, Haartman Institute, University of Helsinki, 
      Haartmaninkatu 3, P.O. Box 21, 00014 Helsinki, Finland. 
      Walter.Koskinen@Helsinki.Fi
FAU - Brondbo, Kjell
AU  - Brondbo K
FAU - Mellin Dahlstrand, Hanna
AU  - Mellin Dahlstrand H
FAU - Luostarinen, Tapio
AU  - Luostarinen T
FAU - Hakulinen, Timo
AU  - Hakulinen T
FAU - Leivo, Ilmo
AU  - Leivo I
FAU - Molijn, Anco
AU  - Molijn A
FAU - Quint, Wim G
AU  - Quint WG
FAU - Roysland, Tov
AU  - Roysland T
FAU - Munck-Wikland, Eva
AU  - Munck-Wikland E
FAU - Makitie, Antti A
AU  - Makitie AA
FAU - Pyykko, Ilmari
AU  - Pyykko I
FAU - Dillner, Joakim
AU  - Dillner J
FAU - Vaheri, Antti
AU  - Vaheri A
FAU - Aaltonen, Leena-Maija
AU  - Aaltonen LM
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20070508
PL  - Germany
TA  - J Cancer Res Clin Oncol
JT  - Journal of cancer research and clinical oncology
JID - 7902060
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Alcohol Drinking/*adverse effects
MH  - Carcinoma, Squamous Cell/*etiology
MH  - DNA, Viral
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*etiology
MH  - Male
MH  - Middle Aged
MH  - Papillomaviridae
MH  - Papillomavirus Infections/*physiopathology
MH  - Polymerase Chain Reaction
MH  - Risk Factors
MH  - Smoking/*adverse effects
EDAT- 2007/05/09 09:00
MHDA- 2007/12/28 09:00
CRDT- 2007/05/09 09:00
PHST- 2006/08/28 00:00 [received]
PHST- 2007/03/23 00:00 [accepted]
PHST- 2007/05/09 09:00 [pubmed]
PHST- 2007/12/28 09:00 [medline]
PHST- 2007/05/09 09:00 [entrez]
AID - 10.1007/s00432-007-0219-8 [doi]
PST - ppublish
SO  - J Cancer Res Clin Oncol. 2007 Sep;133(9):673-8. doi: 10.1007/s00432-007-0219-8. 
      Epub 2007 May 8.