PMID- 17487155
OWN - NLM
STAT- MEDLINE
DCOM- 20071030
LR  - 20220316
IS  - 1750-2799 (Electronic)
IS  - 1750-2799 (Linking)
VI  - 1
IP  - 4
DP  - 2006
TI  - Detection of apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP 
      nick-end labeling and acridine orange in Drosophila embryos and adult male 
      gonads.
PG  - 1725-31
AB  - In Drosophila, vast numbers of cells undergo apoptosis during normal development. 
      In addition, excessive apoptosis can be induced in response to a variety of 
      stress or injury paradigms, including DNA damage, oxidative stress, nutrient 
      deprivation, unfolded proteins and mechanical tissue damage. Two of the most 
      commonly used methods to label apoptotic cells in Drosophila are terminal 
      deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) for fixed 
      tissues and acridine orange (AO) staining for live embryos or tissues. Here, we 
      describe protocols for labeling apoptotic cells in Drosophila embryos and adult 
      male gonads. Slightly modified protocols can also be applied for other Drosophila 
      tissues. The AO protocol is quick, simple and allows real-time imaging of doomed 
      cells in live tissues. However, it is difficult to combine with conventional 
      counterstains or Ab labeling. On the other hand, this functionality is readily 
      afforded by the TUNEL protocol, which permits the detection of apoptotic cells in 
      fixed tissues. These staining procedures can be completed in 1-2 d.
FAU - Arama, Eli
AU  - Arama E
AD  - Howard Hughes Medical Institute, Strang Laboratory of Cancer Research, The 
      Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.
FAU - Steller, Hermann
AU  - Steller H
LA  - eng
GR  - R01 GM60124/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nat Protoc
JT  - Nature protocols
JID - 101284307
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Drosophila/embryology/enzymology/*physiology
MH  - Gonads/physiology
MH  - In Situ Nick-End Labeling/*methods
MH  - Male
EDAT- 2007/05/10 09:00
MHDA- 2007/10/31 09:00
CRDT- 2007/05/10 09:00
PHST- 2007/05/10 09:00 [pubmed]
PHST- 2007/10/31 09:00 [medline]
PHST- 2007/05/10 09:00 [entrez]
AID - nprot.2006.235 [pii]
AID - 10.1038/nprot.2006.235 [doi]
PST - ppublish
SO  - Nat Protoc. 2006;1(4):1725-31. doi: 10.1038/nprot.2006.235.