PMID- 17487350
OWN - NLM
STAT- MEDLINE
DCOM- 20070712
LR  - 20070509
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 30
IP  - 6
DP  - 2007 Jun
TI  - Immunotherapy for malignant tumors using combination of allogeneic intra-bone 
      marrow-bone marrow transplantation, donor lymphocyte infusion and dendritic 
      cells.
PG  - 1309-15
AB  - We have previously shown that the combination of allogeneic intra-bone 
      marrow-bone marrow transplantation (IBM-BMT) and donor lymphocyte infusion (DLI) 
      using CD4+ cell-depleted spleen cells is effective in suppressing tumor growth, 
      but that this does not induce graft-versus-host disease (GVHD) in mice. In this 
      report, we show that formalin-fixed tumor cell-pulsed dendritic cells (FFTCP DCs) 
      have an additive effect with IBM-BMT plus DLI on the suppression of tumor growth, 
      but that the DCs do not augment GVHD. BALB/c mice, which had been subcutaneously 
      inoculated with Meth A (BALB/c-derived fibrosarcoma), were irradiated at a low 
      dose (5 Gy) and were transplanted with bone marrow cells (BMCs) from C57BL/6 (B6) 
      mice into the bone marrow cavity (IBM-BMT). Simultaneously, the mice were 
      intravenously injected with spleen cells from B6 mice, and subcutaneously 
      injected with FFTCP DCs derived from the bone marrow (BM) of B6 mice. At the 
      point of the induction of DCs from BMCs, formalin-fixed Meth A cells were added 
      into the culture. The mice treated with the combination of FFTCP DCs, IBM-BMT and 
      DLI using CD4+ cell-depleted spleen cells showed smaller tumor sizes and longer 
      survival than the mice treated with IBM-BMT plus FFTCP DCs or IBM-BMT plus DLI 
      using CD4+ cell-depleted spleen cells. These results suggest that the combination 
      of FFTCP DCs, IBM-BMT plus DLI using CD4+ cell-depleted spleen cells has potent 
      anti-tumor effects without showing GVHD.
FAU - Mukaide, Hiromi
AU  - Mukaide H
AD  - First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka, 
      Japan.
FAU - Adachi, Yasushi
AU  - Adachi Y
FAU - Koike-Kiriyama, Naoko
AU  - Koike-Kiriyama N
FAU - Suzuki, Yasuhiro
AU  - Suzuki Y
FAU - Minamino, Keizo
AU  - Minamino K
FAU - Iwasaki, Masayoshi
AU  - Iwasaki M
FAU - Tsuda, Masanobu
AU  - Tsuda M
FAU - Nakano, Keiji
AU  - Nakano K
FAU - Koike, Yasushi
AU  - Koike Y
FAU - Shigematsu, Akio
AU  - Shigematsu A
FAU - Kamiyama, Yasuo
AU  - Kamiyama Y
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation
MH  - Dendritic Cells/*transplantation
MH  - Graft vs Host Disease/prevention & control
MH  - Immunotherapy/*methods
MH  - Lymphocyte Transfusion/*methods
MH  - Mice
MH  - Neoplasms, Experimental/*therapy
MH  - *Transplantation Conditioning
MH  - Transplantation, Homologous
EDAT- 2007/05/10 09:00
MHDA- 2007/07/13 09:00
CRDT- 2007/05/10 09:00
PHST- 2007/05/10 09:00 [pubmed]
PHST- 2007/07/13 09:00 [medline]
PHST- 2007/05/10 09:00 [entrez]
PST - ppublish
SO  - Int J Oncol. 2007 Jun;30(6):1309-15.