PMID- 17488685
OWN - NLM
STAT- MEDLINE
DCOM- 20070716
LR  - 20231213
IS  - 1592-8721 (Electronic)
IS  - 0390-6078 (Linking)
VI  - 92
IP  - 5
DP  - 2007 May
TI  - Characterization of ABL1 expression in adult T-cell acute lymphoblastic leukemia 
      by oligonucleotide array analysis.
PG  - 619-26
AB  - BACKGROUND AND OBJECTIVES: Recent data have highlighted an involvement of ABL1 in 
      T-cell acute lymphoblastic leukemia (T-ALL). Specifically, the presence of a 
      fusion gene involving ABL1 and NUP214, both located at 9q34, has been reported. 
      We sought to evaluate whether T-ALL patients with overexpression of ABL showed a 
      peculiar gene expression pattern and were characterized by having specific 
      rearrangements. DESIGN AND METHODS: We previously assessed the expression profile 
      of 128 adults with ALL by oligonucleotide arrays: 33 had T-ALL. In the current 
      study, we evaluated the expression levels of ABL1 in T-ALL cases and found three 
      patients who had ABL1 levels comparable to those detected in BCR/ABL (+)cases and 
      one who had a significantly higher level of ABL1 expression. In order to 
      establish the incidence of ABL1 overexpression in T-ALL, we evaluated 17 
      additional patients by quantitative (Q)-polymerase chain reaction (PCR) and 
      reverse transcription (RT)-PCR. RESULTS: The three cases with ABL1 expression 
      levels comparable to those found in BCR/ABL (+)cases had a specific signature 
      characterized by a high expression of genes involved in regulation of 
      transcription. The fourth case, with the highest levels of ABL, harbored the 
      NUP214-ABL1 rearrangement, which was confirmed by fluorescence in situ 
      hybridization (FISH). Three of the four patients were refractory to induction 
      chemotherapy. Of the 17 additional patients evaluated by Q-PCR and RT-PCR, none 
      showed ABL1 overexpression. INTERPRETATION AND CONCLUSIONS: Overall, 
      overexpression of ABL1 was found in 8% of T-ALL cases. These results underline 
      the value of microarray analyses for the identification of specific signatures 
      associated with ABL1 overexpression, as well as rearrangements, e.g. NUP214-ABL1, 
      in adult T-ALL.
FAU - Chiaretti, Sabina
AU  - Chiaretti S
AD  - Division of Hematology, Department of Cellular Biotechnologies and Hematology, 
      University La Sapienza, Rome, Italy.
FAU - Tavolaro, Simona
AU  - Tavolaro S
FAU - Ghia, Emanuela Maria
AU  - Ghia EM
FAU - Ariola, Cristina
AU  - Ariola C
FAU - Matteucci, Caterina
AU  - Matteucci C
FAU - Elia, Loredana
AU  - Elia L
FAU - Maggio, Roberta
AU  - Maggio R
FAU - Messina, Monica
AU  - Messina M
FAU - Ricciardi, Maria Rosaria
AU  - Ricciardi MR
FAU - Vitale, Antonella
AU  - Vitale A
FAU - Ritz, Jerome
AU  - Ritz J
FAU - Mecucci, Cristina
AU  - Mecucci C
FAU - Guarini, Anna
AU  - Guarini A
FAU - Foa, Robin
AU  - Foa R
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
RN  - 0 (Chromosomal Proteins, Non-Histone)
RN  - 0 (DEK protein, human)
RN  - 0 (EML1-ABL1 fusion protein, human)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (NUP214-ABL1 fusion protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Poly-ADP-Ribose Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (STIL protein, human)
RN  - 0 (TLX3 protein, human)
RN  - 143275-75-6 (TLX1 protein, human)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-abl)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Chromosomal Proteins, Non-Histone/biosynthesis/genetics
MH  - Clinical Trials as Topic/statistics & numerical data
MH  - Female
MH  - Fusion Proteins, bcr-abl/biosynthesis/genetics
MH  - *Gene Expression Profiling
MH  - *Gene Expression Regulation, Leukemic
MH  - *Genes, abl
MH  - Homeodomain Proteins/biosynthesis/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Intracellular Signaling Peptides and Proteins/genetics
MH  - Leukemia-Lymphoma, Adult T-Cell/blood/*genetics
MH  - Male
MH  - Multicenter Studies as Topic/statistics & numerical data
MH  - Neoplasm Proteins/*biosynthesis/genetics
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotide Array Sequence Analysis
MH  - Oncogene Proteins/biosynthesis/genetics
MH  - Oncogene Proteins, Fusion/*biosynthesis/genetics
MH  - Poly-ADP-Ribose Binding Proteins
MH  - Polymerase Chain Reaction
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics/metabolism
MH  - Proto-Oncogene Proteins/biosynthesis/genetics
MH  - Proto-Oncogene Proteins c-abl/*biosynthesis
MH  - RNA, Messenger/biosynthesis/genetics
MH  - RNA, Neoplasm/biosynthesis/genetics
EDAT- 2007/05/10 09:00
MHDA- 2007/07/17 09:00
CRDT- 2007/05/10 09:00
PHST- 2007/05/10 09:00 [pubmed]
PHST- 2007/07/17 09:00 [medline]
PHST- 2007/05/10 09:00 [entrez]
AID - 10.3324/haematol.10865 [doi]
PST - ppublish
SO  - Haematologica. 2007 May;92(5):619-26. doi: 10.3324/haematol.10865.