PMID- 17490435
OWN - NLM
STAT- MEDLINE
DCOM- 20071109
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 122
IP  - 2
DP  - 2007 Oct
TI  - Glycogen synthase kinase 3 activity during development of bone marrow-derived 
      dendritic cells (DCs) essential for the DC function to induce T helper 2 
      polarization.
PG  - 189-98
AB  - Dendritic cells (DCs) polarize naive CD4(+) T cells to either T helper 1 (Th1) or 
      Th2 cells. We examined the role of glycogen synthase kinase 3 (GSK3) activity 
      during DC development from murine bone marrow (BM) cells. DCs were generated by 
      culturing lineage-marker-negative BM cells with granulocyte-macrophage 
      colony-stimulating factor in the presence or absence of a specific inhibitor of 
      GSK3 (Gi), SB415286, for 6 days. DCs generated in the presence (GiDC) or absence 
      (control DC) of SB415286 similarly exhibited a conventional DC phenotype 
      (CD11b(+) B220(-) CD8(-)). These DCs were mixed with allogeneic CD4(+) T cells 
      and the ability to polarize Th1 or Th2 cells was evaluated. The GiDCs exhibited 
      markedly impaired function to induce Th2 polarization compared to control DCs. In 
      contrast, the ability of GiDCs to generate Th1 cells was slightly higher than 
      that of control DCs. CD86 expression and CD40-mediated interleukin-6 production 
      were completely diminished in GiDCs, which might be associated with the impaired 
      ability of the GiDCs to induce Th2 differentiation. These results suggest that 
      the GSK3 activity during DC development is essential for the establishment of the 
      DC function to induce Th2, but not Th1, differentiation.
FAU - Ono, Takenori
AU  - Ono T
AD  - Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University, 
      Sapporo, Japan.
FAU - Yanagawa, Yoshiki
AU  - Yanagawa Y
FAU - Iwabuchi, Kazuya
AU  - Iwabuchi K
FAU - Nonomura, Katsuya
AU  - Nonomura K
FAU - Onoe, Kazunori
AU  - Onoe K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070509
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (3-(3-chloro-4-hydroxyphenylamino)-4-(4-nitrophenyl)-1H-pyrrole-2,5-dione)
RN  - 0 (Aminophenols)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD3 Complex)
RN  - 0 (CD40 Antigens)
RN  - 0 (Cytokines)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Maleimides)
RN  - 0 (Toll-Like Receptors)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
SB  - IM
MH  - Aminophenols/pharmacology
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antigen Presentation/immunology
MH  - Bone Marrow Cells/enzymology
MH  - CD3 Complex/immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD40 Antigens/immunology
MH  - Cell Differentiation/immunology
MH  - Cell Proliferation
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/*enzymology/immunology
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Glycogen Synthase Kinase 3/antagonists & inhibitors/immunology/*metabolism
MH  - Immunophenotyping
MH  - Lymphocyte Activation/immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Maleimides/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Th2 Cells/*immunology
MH  - Toll-Like Receptors/immunology
PMC - PMC2266003
EDAT- 2007/05/11 09:00
MHDA- 2007/11/10 09:00
PMCR- 2008/10/01
CRDT- 2007/05/11 09:00
PHST- 2007/05/11 09:00 [pubmed]
PHST- 2007/11/10 09:00 [medline]
PHST- 2007/05/11 09:00 [entrez]
PHST- 2008/10/01 00:00 [pmc-release]
AID - IMM2627 [pii]
AID - 10.1111/j.1365-2567.2007.02627.x [doi]
PST - ppublish
SO  - Immunology. 2007 Oct;122(2):189-98. doi: 10.1111/j.1365-2567.2007.02627.x. Epub 
      2007 May 9.