PMID- 17490513
OWN - NLM
STAT- MEDLINE
DCOM- 20071030
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 15
IP  - 1
DP  - 2007 Feb
TI  - [Detection of rearrangements of mixed lineage leukemia gene and its clinical 
      significance].
PG  - 20-4
AB  - To study the incidence, the types of fusion genes and the clinical significance 
      of rearrangements of mixed lineage leukemia (MLL) gene in acute leukemia (AL), 
      the rearrangements of MLL gene of 60 patients with AL were detected by 
      fluorescence in situ hybridization (FISH) and 6 types of common fusion genes 
      resulting from the rearrangements of MLL gene were detected by nested RT-PCR. The 
      results showed that 7 out of 60 AL patients were found the rearrangements of MLL 
      gene, the incidence of which was 11.67%. 2 out of 7 patients were diagnosed as 
      AML-M(5), 5 patients were diagnosed as B-ALL. The fusion genes of the 2 AML-M(5) 
      patients who had the rearrangements of MLL gene were MLL/AF(9). Among 5 B-ALL 
      patients, 2 patients were confirmed to express MLL/ENL, 1 patient was confirmed 
      to express MLL/AF(4), the other 2 patients did not express the fusion genes. It 
      is concluded that FISH is a fast, specific and sensitive method to detect the 
      rearrangements of MLL gene in AL patients and nested RT-PCR is a convenient and 
      feasible method to detect the types of fusion genes resulting from the 
      rearrangements of MLL gene. The detection of MLL gene rearrangement is of great 
      importance in predicting prognosis and guiding therapy in AL.
FAU - Chen, Wei-Hong
AU  - Chen WH
AD  - Department of Hematology, The Second People Hospital of Shenzhen, Shenzhen 
      Institute of Hematology, Shenzhen 518035, China.
FAU - Chen, Cheng-Jian
AU  - Chen CJ
FAU - Wang, Ming-Chun
AU  - Wang MC
FAU - Li, Chang-Gang
AU  - Li CG
FAU - You, Wei-Wen
AU  - You WW
FAU - Huang, Rui-Hong
AU  - Huang RH
FAU - Li, Ming
AU  - Li M
FAU - Tao, Xiao-Mei
AU  - Tao XM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - Middle Aged
MH  - Myeloid-Lymphoid Leukemia Protein/*genetics
MH  - Oncogene Proteins, Fusion/genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
EDAT- 2007/05/11 09:00
MHDA- 2007/10/31 09:00
CRDT- 2007/05/11 09:00
PHST- 2007/05/11 09:00 [pubmed]
PHST- 2007/10/31 09:00 [medline]
PHST- 2007/05/11 09:00 [entrez]
AID - 1009-2137(2007)01-0020-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Feb;15(1):20-4.