PMID- 17490521
OWN - NLM
STAT- MEDLINE
DCOM- 20071030
LR  - 20181201
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 15
IP  - 1
DP  - 2007 Feb
TI  - [Influence of Interferon alpha-2b on proliferation inhibition and apoptosis 
      induction in HL-60 cells].
PG  - 56-8
AB  - To investigate the effects of interferon alpha-2b on proliferation and apoptosis 
      in HL-60 cells, HL-60 cells were cultured in different concentrations of IFN 
      alpha-2b. The morphologic changes were observed by Wright's and acridine orange 
      (AO) and ethidium bromide (EB) staining respectively. Inhibition of proliferation 
      was detected by MTT. Expression of CD13(+) was checked by indirect 
      fluoroimmunoassay. The results showed that apoptosis rate of HL-60 cells assayed 
      by the above-mentioned two methods was (51 +/- 2)% and (78 +/- 3)% respectively 
      and OD(570) values of proliferation inhibited were 1.8 +/- 0.1 and 1.0 +/- 0.1 
      respectively when the concentrations of the IFN(alpha-2b) were 500 and 10,000 
      U/ml in culture for 48 hours. Morphology and count of CD13(+) cells were changed. 
      CD13(+) cell expression rate was (62 +/- 2)% and (30 +/- 3)% respectively when 
      the concentrations of the IFN(alpha-2b) were 500 and 10,000 U/ml in culture for 
      48 hours. It is concluded that IFN(alpha-2b) can enhance the apoptosis of HL-60 
      cells, inhibit their proliferation, promote their maturation and differentiation.
FAU - Yu, Hong
AU  - Yu H
AD  - Department of Pediatrics, Affiliated Hospital of Medical College, Qingdao 
      University, Qingdao 266003, China.
FAU - Sun, Li-Rong
AU  - Sun LR
FAU - Pang, Xiu-Ying
AU  - Pang XY
FAU - Li, Xue-Rong
AU  - Li XR
FAU - Lu, Yuan
AU  - Lu Y
FAU - Song, Ai-Qin
AU  - Song AQ
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Interferon alpha-2)
RN  - 0 (Interferon-alpha)
RN  - 0 (Recombinant Proteins)
RN  - EC 3.4.11.2 (CD13 Antigens)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/*drug effects
MH  - CD13 Antigens/*biosynthesis/genetics
MH  - Cell Proliferation/drug effects
MH  - HL-60 Cells
MH  - Humans
MH  - Interferon alpha-2
MH  - Interferon-alpha/*pharmacology
MH  - Recombinant Proteins
EDAT- 2007/05/11 09:00
MHDA- 2007/10/31 09:00
CRDT- 2007/05/11 09:00
PHST- 2007/05/11 09:00 [pubmed]
PHST- 2007/10/31 09:00 [medline]
PHST- 2007/05/11 09:00 [entrez]
AID - 1009-2137(2007)01-0056-03 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Feb;15(1):56-8.